Fig. 1. PAR-6 localization in epithelial cells. In this and all subsequent figures, embryos (50 µm in length) or embryo insets are oriented anterior to the left. Nuclei are stained with DAPI (blue) and show cell-type differences in staining intensity. (A,B) Lateral views of embryos when epithelial cells are polarizing (A, bean stage) or after most epithelial cells have completed polarization (B, 1.5 fold stage). PAR-6 localizes to apical surfaces of cells in each of the major epithelia (labeled); staining in epidermal epithelial cells is weaker and more difficult to detect in older embryos (compare A and B). -PAR-6 antibodies also recognize germline P granules (P). (A',B') Boxed regions magnified in insets below also show PAR-3 staining; inset contrast has been adjusted to indicate nuclei. (C-H) Dorsoventral views of polarizing intestinal cells showing PAR-6^GFP and junction proteins. The intestinal midline is indicated with opposing arrowheads. (C-E) PAR-6^GFP and HMP-1/-catenin at initial stages of intestinal cell polarization (before apical migration of intestinal nuclei). (F-H) PAR-6^GFP and DLG-1 in polarizing intestinal cells (after apical migration of intestinal nuclei). Scale bars: 2.5 µm.