Development -- Totong et al. 134 (7): 1259 Figure IG2

(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.

Fig. 2. PAR-6^ZF1GFP localization and degradation. Panels show par-6(zu170) embryos expressing maternal PAR-6^ZF1GFP stained with ${alpha}$ -PAR-6 antibodies (green) and/or ${alpha}$ -GFP antibodies (red). ${alpha}$ -PAR-6 antibodies also react non-specifically with P granules (P). (A,B) One-cell embryo co-stained for PAR-6 and GFP; PAR-6^ZF1GFP is at the anterior cortex (arrow). (C) A 26-cell embryo; PAR-6^ZF1GFP has degraded from most cells except the germ-line precursor cell (asterisk) and the youngest somatic cells (arrows). (D) $~$ 100-cell embryo; PAR-6^ZF1GFP is detectable only in the two germ cells (asterisks). (E,F) 1.25 fold stage embryos; PAR-6 (arrow in E) but not PAR-6^ZF1GFP (F) is detectable in epithelial cells. Scale bar: 2.5 µm.