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Figure 3


Fig. 3. The par-6(tm1425) deletion and PAR protein localization in par-6(M/Z) embryos. (A) The par-6 gene (top) and its predicted full-length product (bottom). Exons (rectangles), introns (lines), and the region deleted in tm1425 are indicated. The PB1, CRIB (C) and PDZ domains are shown in the predicted protein, and sequences encoding these domains are colored in par-6 exons. (B,C) PAR-6, PKC-3, and PAR-3 localization in wild-type (B) and par-6(M/Z) (C) embryos. (B',C') Magnifications of the boxed regions in B,C. PKC-3 is uniform in par-6(M/Z) embryos. Nonspecific staining of P granules by PAR-6 antibodies is indicated (P). Scale bars: 2.5 µm.