Fig. 6. Apical junctions in par-6(M/Z) embryos. Wild-type or par-6(M/Z) embryos immunostained as indicated. Dashed boxes are magnified in the insets below; the contrast within insets has been increased to highlight intestinal epithelial cell staining. Nonspecific staining of P granules by PAR-6 antiserum is indicated (P). (A,B) PAR-6 and HMP-1. In wild-type (A), PAR-6 is apical and HMP-1 is present in more lateral junctions. HMP-1 is apical but fragmented in par-6(M/Z) embryos (B). (C,D) HMR-1 and DLG-1. In wild type (C), HMR-1 and DLG-1 are present together in apical junctions within adjacent domains. In par-6(M/Z) embryos (D), HMR-1 and HMP-1 are apical but fragmented and do not colocalize (arrow, D'). (E,F) LET-413 and PAR-3 in epidermal cells, lateral view in bean stage embryos. In both wild-type (E) and par-6(M/Z) (F) embryos, LET-413 is found at lateral surfaces (arrows) but not apical surfaces (arrowheads); PAR-3 is present only at the apicolateral interface. Scale bars: 2.5 µm.