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Figure 7


Fig. 7. DLG-1 localization during junction formation. (A,B) Fluorescence timelapse movie stills (taken from Movies 1, 2 in the supplementary material) of wild-type (A) and par-6(M/Z) (B) epidermal cells expressing DLG-1GFP. Times are indicated in minutes:seconds, with 00:00 representing the time DLG-1GFP is first expressed in epidermal cells. DLG-1GFP forms continuous junctions in wild-type but not par-6(M/Z) embryos. (C,D) DLG-1 and PKC-3 localization in intestinal epithelial cells of wild-type (C) and par-6(M/Z) (D) embryos. Embryos are at the 1.25 fold stage, just after DLG-1 organizes into continuous junctions in wild-type embryos. DLG-1 in par-6(M/Z) embryos is always fragmented. Scale bars: 2.5 µm.