Fig. 5. opa LOF clones fail to express dpp^s-hc-lacZ. Homozygous opa mutant clones were generated by using the FLP/FRT system. dpp^s-hc-lacZ construct, SH53, expression (in red) in non-recombination control disc (A), and in opa mutant tissue in single section images (B-E). The clonal areas are outlined in white in the merged panels (B-D) and are marked by absence of green fluorescence, which can be seen in the B'-E' green-only channel. The box in E indicates dpp reporter expression missing in a small clone, and its enlargement is shown in the white inset box in E and E'. The positions of clones relative to the entire lateral dpp^s-hc-lacZ expression pattern is shown by labeled brackets in A.