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Figure 1


Fig. 1. The construct for transgenic activation of Pax6. The construct pJoP6 contains the ß-actin/CMV fusion promotor and a loxP-flanked gfp-stop cassette placed in front of the Pax6 and lacZ coding sequences. After Cre-mediated recombination, the gfp-stop cassette is eliminated allowing simultaneous expression of transgenic Pax6 and lacZ via an IRES sequence from the resulting construct pJoP6rec. Arrows indicate the annealing sites of the primers JoP6F and JoP6R.