Fig. 3. Most Notch was not co-localized with ER markers in O-fut1^- cells. (A-E) O-fut1^- somatic clones (labeled O-fut1^-) generated in wing discs expressing ER marker PDI-GFP (A-C) or ER-CFP (driven by sd-GAL4; D,E), and stained with anti-Notch (C17.9C6, magenta) and anti-GFP (green) antibodies. (A) Normal confocal image showing the subapical region of the wing disc epithelium, where Notch accumulated in O-fut1^- cells. (B) The resolution of A was improved using deconvolution. (C) Optical vertical section of B. (D) Deconvolution was used to improve the resolution of this image. (E) Optical vertical section of D. (F) A positive control for deconvolution analysis. Wing discs expressing PDI-GFP were incubated with a rat anti-GFP antibody, and stained simultaneously with two anti-rat secondary antibodies, conjugated to Alexa 488 or Cy3. All wing discs were isolated from late third-instar larvae. Right panels show merged images of the left and middle panels. Higher magnifications of O-fut1^- cells are shown in the small panels at right (B-F). Clone boundaries are indicated by white dashed lines (A-E).