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Figure 1


Fig. 1. Expression of ced-3 promoter::GFP transgenes is induced shortly before tail-spike cell death. (A) Cartoon showing the tail-spike cell at different stages of development. Filled green ovals, tail-spike cell nuclei; gray oval, dying tail-spike cell corpse. Ball-stage embryo (left); threefold-stage embryo (center and right). (B-D) Merged DIC and GFP images of threefold-stage embryos expressing a 1.5 kb C. elegans ced-3 promoter::GFP reporter transgene (nsEx723). (B) Embryo before the onset of tail-spike cell GFP expression (arrow and inset: binucleate tail-spike cell). (C) Embryo at onset of tail-spike cell GFP expression (arrow and inset: bi-nucleate tail-spike cell). (D) Embryo approximately 31 minutes after the onset of tail-spike cell GFP expression (arrow and inset: dying tail-spike cell corpse). (E) Merged DIC and GFP image of a threefold-stage embryo expressing an egl-1 promoter::GFP integrated transgene (bcIs1). Arrow and inset: tail-spike cell corpse lacking GFP. Scale bars: 10 µm.