Fig. 1. Analysis of the of Ripply2 expression pattern. (A) Comparison of the mRNA expression patterns of Mesp2 and Ripply2 during mouse development. Positive expression is indicated by an arrowhead. (B) Comparison of the spatial expression patterns of Mesp2 and Ripply2 as revealed by section double in situ hybridization. Two representative examples are shown for Mesp2 (green) and Ripply2 (magenta), and merged images of these expression patterns are shown beneath. The green signals in the periphery are artifacts and do not represent Mesp2 expression. In some cases, only a single band could be observed for each gene, and these bands are merged in the image shown in the left-hand bottom panel. Two bands were sometimes visible for Ripply2, the posterior band of which merges with that of Mesp2 (right-hand bottom panel). All samples were prepared from E10.5 embryos. (C) Whole-mount in situ hybridization showing that Ripply2 expression is lost in the E9.5 Mesp2-null embryo.