Fig. 2. Creb^DBHCre mutants show no gross morphological abnormalities in the sympathetic ganglia. Sagittal sections from control (Creb^fl/fl) and mutant (Creb^DBHCre) embryos at E17.5 are stained with anti-CREB antibody (A,B). CREB immunoreactivity is indicated in the SCG (enclosed area) by arrows in neurons, and by arrowheads in other morphologically distinct cells. Nissl staining reveals no major differences between control (C) and mutant (D). Whole-mount immunohistochemistry with TH antibody of P2 control (E) and Creb^DBHCre (F) animals reveals no major deficits in ophthalmic projections (arrows). e, eye. Scale bar: 100 µm in A,B; 50 µm in C,D; 500 µm in E,F.