Fig. 1. Recombinant laminin that contains dystroglycan-binding, but not integrin-binding, sites binds to the surface of differentiated oligodendrocytes. (A) rE3 is a recombinant laminin protein that comprises the last two LG domains of the laminin -subunit. rE3 has been shown previously to contain dystroglycan-binding, but not integrin-binding, sites. Oligodendrocytes were differentiated for the indicated times in the presence or absence of 10 µg/ml rE3, washed to remove unbound protein and lysed. Western blots of lysates were performed to detect the presence of rE3. Blots were reprobed with actin antibodies as a loading control. (B) Densitometry to determine the average relative intensity of the captured rE3 signal (n=3, ^**P<0.01, error bars represent s.d.). (C) FLAG antibodies followed by FITC-conjugated secondary antibodies were used to detect cell-surface binding of rE3 to differentiated oligodendrocytes. Control (ctrl) image depicts FLAG immunofluorescence on cells without added rE3. Representative merged phase and fluorescent micrographs are shown.