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Figure 3


Fig. 3. Oligodendrocytes differentiated on laminin show increased levels of dystroglycan. (A) Relative dystroglycan expression in oligodendrocytes differentiated for 1 or 4 days on poly-D-lysine (PDL) or laminin (Lm). Western blots were probed with antibodies specific for {alpha}-dystroglycan ({alpha}-DG) or ß-dystroglycan (ß-DG). Blots were reprobed with antibodies against actin as a loading control. A representative western blot is shown. (B) Densitometry to determine relative {alpha}-DG expression. The average fold increase relative to expression at day 1 on PDL is shown (n=3, *P<0.05 and **P<0.01, error bars represent s.d.). (C) Densitometry to determine relative ß-DG expression. The average fold increase relative to expression at day 1 on PDL is shown (n=3, *P<0.05, error bars represent s.d.). ND, no significant difference.