Fig. 2. Decreased proliferation of cortical progenitors after Par3 knockdown. (A-D) Fluorescence micrographs depicting a two-cell clone generated from an E12 mouse cortical progenitor after Par3 shRNA transduction, immunolabeled for GFP (green), MAP2 (red) and Tbr1 (blue). Note that both GFP-positive cells express the neuronal markers MAP2 or Tbr1 (arrows). (E) Bar chart representing the frequency of control and Par3b shRNA-transduced cell clones according to the size (number of cells per clone, x-axis). Asterisks indicate statistically significant differences between control (n=80 clones) and Par3 shRNAs (Par3a, n=40; Par3b, n=87; Par3c, n=57 clones) for each clone size (^*, P<0.05; ^**, P<0.01; Tukey's multiple comparison test). Note the lack of clones larger than 10 cells after Par3a shRNA-transduction. (F,G) Quantification of the mean number of GFP-positive cells per clone (F) and the proportion of neurons (G) generated after control or Par3 shRNA transduction (^*, P<0.05; ^**, P<0.01; ^***, P<0.001; Tukey's multiple comparison test). Scale bar: 25 µm.