Fig. 7. Par6 overexpression increases the number of Pax6+ progenitors. (A-I) Examples of two-cell clones (pairs) obtained from E12 mouse cortical cells transduced with control (A-F) or Par6 (G-I) cDNA and immunolabeled for GFP (green) and Pax6 (red) after 2 days in vitro (2div). White arrows point to Pax6/GFP double-positive cells and black arrows to GFP-positive Pax6 negative cells. (J) Bar chart depicting the frequency of pairs (control, n=84; Par6, n=59) containing two Pax6-positive cells (black bar), only one Pax6-positive cell (gray), or only Pax6-negative cells (white). (K) Bar chart representing the same quantification as in J but for Tbr2 (control, n=61; Par6, n=54). Note the increased proportion of pairs containing two Pax6-positive cells after Par6 overexpression. By contrast, the frequency of pairs containing two Tbr2-positive cells was significantly reduced after Par6 transduction. Scale bar: 25 µm.