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Figure 1


Fig. 1. Hoxb6-cre activates the R26 reporter gene in lateral plate mesoderm cells before the first expression of the SMC marker SM22{alpha}. (A-C) lacZ staining for the Hoxb6-cre-activated R26 reporter gene at three levels between the prospective forelimb and hindlimb regions at E8.5-E9.0 showed reporter expression in splanchnic (arrowheads) and somatic (arrows) lateral plate mesoderm. The reporter was also expressed in hindgut endoderm. (D,E) In posterior regions of the embryo, Hoxb6-cre had a broader expression domain and the reporter was also expressed in paraxial mesoderm cells (arrowheads in E; E shows boxed area in D) and cells in the neural plate (arrows in E). (F-H) The first expression of the SM22{alpha}-lacZ gene in the forelimb bud region was detected at E9.0-E9.5 in splanchnic mesoderm adjacent to the aorta (arrows in F and G), and in cells situated between the dorsal aortas (arrows in H). d-v, dorsal-ventral axis; nt, neural tube; nf, neural folds; np, neural plate; pm, paraxial mesoderm; da, dorsal aorta; hg, hindgut; va, vitelline artery; c, coelomic cavity. Scale bars: 25 µm.