Fig. 10. PDGFD injection increases opV trigeminal placode size and neurogenesis. (A,C) Control injected section of midbrain at st. 11 shows Pax3 (green) in opV trigeminal placode. White line indicates ventral extent of opV trigeminal placode. (C) Nuclear DAPI (blue) staining of A. (B,D) PDGFD injection (250 ng/µl solution) increases the number of Pax3+ cells and ventral spread of placode (white line). (D) Nuclear DAPI (blue) staining of B. (E) Control injected section of midbrain at st. 15 shows Pax3 (green) in condensing ganglion. (F) Hu/NF (red) expression in same control section. (G) Merge plus DAPI (blue) in same control section. (H,I) PDGFD injection results in increased Pax3+ (green) cells (H) and neurogenesis as detected with Hu/NF (red) (I) in condensing opV trigeminal ganglion. (J) Merge plus DAPI (blue) in same PDGFD injected section. (K) PDGFD injection increases Pax3+ cells by 32% at st. 11 and Pax3/Hu/NF+ cells by 54% compared with controls (st. 11: control n=8, PDGFD n=10; st. 15: control n=6, PDGFD n=6). Error bars are s.e.m. ^*P<0.004. Arrows indicate Pax3+ cells (A-D), or Pax3/Hu/NF+ opV trigeminal neuron (E-J).