Fig. 6. Lgl interacts with Par-1 and is required for Par-1 localization. (A,B) Both the GFP-Par-1(N1S) (A) and GFP-Par-1(N1S) K^* (B) were enriched at the posterior at stage 10. (C,C') The GFP-Par-1(N1S) K^* lost the posterior enrichment in lgl⁴ germline cells. Mutant germline clones were marked by the absence of β-gal staining (red) in the nuclei of the nurse cells. (D-D") GFP-Par-1(N1S) colocalized with Lgl and was detected throughout the oocyte cortex in egg chambers with co-overexpression of Lgl-3A and GFP-Par-1(N1S). Arrows indicate the strong GFP-Par-1 and arrowheads indicate the weak GFP-Par-1. (E) Immunoblots of anti-Lgl or anti-GFP immunoprecipitation from ovaries with GFP-Par-1 (N1S) overexpression (mat-Gal4 driver) or the wild-type control (mat-Gal4 driver only). aPKC and GFP-Par-1 (N1S) proteins were precipitated by the anti-Lgl antibody from GFP-Par-1 (N1S) overexpression, and aPKC and Lgl proteins were precipitated by an anti-GFP antibody from GFP-Par-1 (N1S) overexpression. Input was one-fifth of the total ovarian extract. In anti-Lgl immunoprecipitates, controls were beads (without added antibody) and peptide blocks (+pep); in anti-GFP immunoprecipitates, controls were beads (without added antibody) and ovarian extract from wild-type flies.