Fig. 6. Changed differentiation of chromaffin cells in Insm1 mutant mice. Adrenal glands of Insm1^lacZ/+ and Insm1^lacZ/Insm1^lacZ mice (E14.5) were analyzed by (A,B) X-gal staining, (C,D) in situ hybridization using a Mash1-specific probe and (E,F) immunohistochemistry using antibodies against β-galactosidase (red) and Th (green). Adrenal glands of Insm1^lacZ/+ and Insm1^lacZ/Insm1^lacZ mice (E18.5) were analyzed by (G,H) immunohistochemistry using antibodies against β-galactosidase (red) and PNMT (green), and by in situ hybridization using probes specific for (I,J) Th, (K,L) Dbh, (M,N) chromogranin A and (O,P) chromogranin B. (Q) Quantification of total numbers of β-galactosidase+ cells in adrenal glands at various developmental stages in Insm1^lacZ/+ and Insm1^lacZ/Insm1^lacZ mice. Double asterisks indicate a P<0.01. Scale bars: 100 µm (A,G).