Fig. 4. Flow cytometric analysis of NG2-positive, CD34-positive populations. (A,B) Flow cytometry was used to examine NG2 and CD34 expression by (A) cells recovered from dissociated 5-day Matrigel plugs and (B) cells in total adult bone marrow. In A, 8.2% of the Matrigel population expressed NG2 alone, 6.6% expressed CD34 alone and 74.2% expressed both markers. In B, 5.3% of the total bone marrow population expressed NG2 alone, 8.1% expressed CD34 alone and 1.7% expressed both markers. In control experiments with second antibodies alone, fewer than 0.5% of cells were scored as positive.