Fig. 6. Bone marrow origin of vascular cells. Matrigel plugs were established in irradiated mice that had been reconstituted using bone marrow cells from an EGFP⁺ donor. At day 5 (A-D) sections were analyzed by staining for NG2 (magenta), CD34 (red) and DAPI (blue). The EGFP marker (green) is seen in cells that co-express NG2 and CD34 (arrows). Also at day 5 (E-H), NG2 (magenta), F4/80 (red), EGFP (green) and DAPI (blue) labeling were compared. The EGFP label is apparent in both populations of macrophages: F4/80⁺NG2⁺ (arrows) and F4/80⁺NG2^- (arrowheads). At day 9 (I-L), sections were analyzed for NG2 (magenta), CD31 (red), EGFP (green) and DAPI (blue). The EGFP marker is frequently found in NG2⁺ cells (arrowheads), and more rarely in CD31⁺ cells (arrows). All panels represent z-stacks of confocal images. Scale bars: 40 µm.