Fig. 8. Oligodendrocyte marker gene expression in Sox9^loxP/loxP, Sox10^lacZ/lacZ, Sox10::Cre spinal cords. (A-D) Quantification of oligodendroglial marker gene expression at 18.5 dpc as the percentage of Olig2-positive cells that also expressed NG2 (A), DCC (B), O4 (C) and Nkx2.2 (D) in wild-type (white bars), Sox10^lacZ/lacZ (Sox10^-/-; dark grey bars) and Sox9^loxP/loxP, Sox10^lacZ/lacZ, Sox10::Cre (Sox9^/, Sox10^-/-; black bars) spinal cords at 18.5 dpc following immunocytochemistry on acutely dissociated spinal cord cultures (A-C) or immunohistochemistry on transverse spinal cord sections (D). For all quantifications, at least 10 separate cover slips or 30 separate 10 µm sections from the forelimb region were counted from two independent embryos for each genotype. Data are presented as mean±s.e.m. Differences from the wild-type were statistically significant for Nkx2.2 in Sox9^loxP/loxP, Sox10^lacZ/lacZ, Sox10::Cre spinal cords, as determined by Student's t-test (^***P0.001). (E) Co-immunohistochemistry with antibodies specific for Olig2 (in red) and Nkx2.2 (in green) were performed on transverse sections from the forelimb region of wild-type, Sox10^lacZ/lacZ and Sox9^loxP/loxP, Sox10^lacZ/lacZ, Sox10::Cre spinal cords at 18.5 dpc. Comparable regions of the ventral marginal zone are shown. (F) In situ hybridization with a Plp-specific probe was performed on transverse sections from the forelimb region of wild-type, Sox10^lacZ/lacZ and Sox9^loxP/loxP, Sox10^lacZ/lacZ, Sox10::Cre spinal cords at 18.5 dpc.