Fig. 2. Rbsn-5 is required for osk RNP localization and the maintenance of MT polarity. (A,C,E) Stau localization in stage 6-10 Drosophila oocytes of wild type (A), rbsn-5^- GLC (C) and rbsn-5^- GLC expressing the rbsn-5 transgene (E). Arrowheads point to mislocalized Stau signals in rbsn-5^- oocytes. F-actin was counterstained with Alexa-660-conjugated phalloidin (cyan). (B,D,F) Osk localization in stage 10 oocytes of wild type (B), rbsn-5^- GLC (D) and rbsn-5^- GLC expressing the rbsn-5 transgene (F). DNA stained with DAPI is shown in cyan. (G-J) In situ hybridization for osk (green) and grk or bcd (magenta) RNAs in wild-type (G,H) or rbsn-5^- GLC (I,J) stage 9 oocytes. DNA stained with DAPI is shown in grayscale. In rbsn-5^- oocytes, osk RNA localization was disrupted (arrows), but grk or bcd RNA localization was unaffected (arrowheads). (K-N) Stau and Kin-βgal distribution in stage 8-9 wild-type (K,L) or rbsn-5^- GLC (M,N) oocytes. Arrowheads in N indicate aberrantly localized Stau and Kin-βgal in the rbsn-5^- GLCs. DNA was counterstained with DAPI (cyan). Scale bars: 20 µm.