Fig. 1. Two cdc-25.1(gf) mutations affect a putative β-TrCP-like phosphodegron, while rr36 corresponds to a new temperature-sensitive lf mutation. Schematic representation of the CDC-25.1 phosphatase highlighting the corresponding amino acid changes: the two gain-of-function mutations within the β-TrCP motif (blue) in the N-terminal regulatory domain and the intragenic rr36 mutation adjacent (N-terminal) to the catalytic phosphatase domains (black and red) (see Materials and methods for description). The amino acid changes are in red. The consensus β-TrCP phosphodegron motif is boxed, with its phosphorylable serines in green.