Fig. 1. Molecular identification of AE5 and AE6. (A,B) Fine structure mapping to localize AE5 and AE6 genes on chromosome 2 (A, upper panel) and 3 (B, upper panel), and AE5 (At2g19730) and AE6 (At3g25520) encode ribosomal proteins RPL28A (A, lower panel) and RPL5A (B, lower panel), respectively. Black and white boxes indicate the protein-coding region and untranslational region (UTR), respectively. (C) RT-PCR shows that AE5 and AE6 were expressed in all plant tissues examined. (D-J) In situ hybridization analyses to determine AE5 (D,F,H) and AE6 (E,G) expression patterns. (D) A late-globular-stage embryo. (E) A heart-stage embryo. (F) A longitudinal section of an 11-day-old seedling. (G) A transverse section of an 11-day-old seedling. (H) A longitudinal section of an inflorescence meristem. (I,J) No hybridization signals were detected either with the AE5 sense probe (I) or with the AE6 antisense probe for tissues from the insertional allele, ae6-2 (J). Blue lines in A,B indicate probe regions used for in situ hybridization. sam, shoot apical meristem; lp, leaf primordium; yl, young leaf; im, inflorescence meristem; fm, floral meristem; fp, flower primordium; s, sepal; st, stamen; c, carpel; an, anther. Scale bars: 25 µm in D,E; 100 µm in F-J.