Fig. 3. Gene-specific RNAi effects in primary cells. Primary cells were isolated from G053 Drosophila embryos expressing the SLS-GFP fusion protein and were treated with dsRNAs targeting lacZ (A,D), sls (B,E) and Mhc (C,F). SLS-GFP expression was detected by GFP (A-C, green in D-F). Muscle structure was revealed by SLS-GFP in green, phalloidin staining of Actin in red, and DAPI staining of nuclei in blue (D,E,F). Scale bar: 20 µm.