spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Smith, E. P.
Right arrow Articles by D'Ercole, A. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Smith, E. P.
Right arrow Articles by D'Ercole, A. J.

Development, Vol 101, Issue 1 73-82, Copyright © 1987 by Company of Biologists

JOURNAL ARTICLES

Somatomedins/insulin-like growth factors, their receptors and binding proteins are present during mouse embryogenesis

EP Smith, TW Sadler and AJ D'Ercole
Department of Pediatrics, School of Medicine, University of North Carolina, Chapel Hill 27514.

Somatomedins/insulin-like growth factors (Sm/IGFs) are considered to have important roles in regulating fetal growth; however, because of limited quantities of tissue, few studies have been performed on their effects on embryonic growth. To assess a potential role for these factors, we evaluated mouse embryonic tissues for the presence of Sm/IGF and insulin receptors and Sm/IGF-binding proteins by chemical affinity labelling. In addition, we measured extractable Sm-C/IGF-I radioimmunoactivity in mouse embryonic tissues. Finally, we compared these data with those from the embryonal carcinoma cell line, PC13. All embryos from day 9 (3-4 somites) to day 12 (45 somites) possessed both Sm-C/IGF-I and IGF-II receptors in apparent greater abundance than insulin receptors. The visceral yolk sac appeared to have proportionally more insulin receptors than the corresponding embryonic tissue. Extracts from the embryos contained immunoreactive Sm-C/IGF-I and binding proteins of 30-45 X 10(3) Mr. PC13 cells possessed all three receptors and the apparent abundance of the insulin and IGF-II receptors was reduced after differentiation was induced with retinoic acid. PC13 cells released both immunoreactive Sm-C/IGF-I- and Sm-C/IGF-I-binding proteins into their medium. When differentiated, the binding proteins resembled the native ones extracted from the intact embryos. The presence of Sm/IGF activity, receptors and binding proteins in early embryogenesis suggests a role for these factors in embryonic growth. The PC13 cell line appears to only partially reflect normal development.


This article has been cited by other articles:


Home page
 Endocr. Rev.Home page
V. C. Russo, P. D. Gluckman, E. L. Feldman, and G. A. Werther
The Insulin-Like Growth Factor System and Its Pleiotropic Functions in Brain
Endocr. Rev., December 1, 2005; 26(7): 916 - 943.
[Abstract] [Full Text] [PDF]

Home page
 Genes Dev.Home page
D A Rappolee, K S Sturm, O Behrendtsen, G A Schultz, R A Pedersen, and Z Werb
Insulin-like growth factor II acts through an endogenous growth pathway regulated by imprinting in early mouse embryos.
Genes & Dev., June 1, 1992; 6(6): 939 - 952.
[Abstract] [PDF]


© The Company of Biologists Ltd 1987