spacer gif spacer gif spacer gif spacer gif ARCHIVE ANNOUNCEMENT! spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Griffin, K. J.
Right arrow Articles by Carlson, B. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Griffin, K. J.
Right arrow Articles by Carlson, B. M.

Development, Vol 101, Issue 2 267-277, Copyright © 1987 by Company of Biologists

JOURNAL ARTICLES

A monoclonal antibody stains myogenic cells in regenerating newt muscle

KJ Griffin, DM Fekete and BM Carlson
MRC Cell Biophysics Unit, King's College London, UK.

Monoclonal antibodies have been used to study minced muscle regeneration in the adult newt, Notophthalmus viridescens. The contralateral limb was amputated and the immunostaining patterns in the regenerating blastema were compared with the minced tissue in sectioned material. Staining with a myofibre-specific antibody, called 12/101 (Kintner & Brockes, 1984), showed that myofibre degeneration was complete by 8-10 days after mincing, with myogenesis commencing 2 days later. Another monoclonal antibody, called 22/18, previously shown to label a subset of cells in the regeneration blastema of the newt (Kintner & Brockes, 1984, 1985), was found also to recognize a population of cells in regenerating minced muscle. At 6 days after mincing, the number of 22/18-positive (22/18+) cells was low but by days 12-16, during the period of myogenesis, their number had increased to become a major population within the minced tissue. A small number of the 22/18+ cells could be double labelled with 12/101 at this time. Prior to this, there was a phase in which 12/101 staining had disappeared from the mince. Cells immunoreactive with both antibodies after this phase confirm that at least some of the 22/18+ cells are myogenic. The number of 22/18+ cells decreased as muscle repair and maturation progressed. These results show that 22/18 is not specifically associated with blastemal cells but is a more general marker for regenerating systems in the newt. They further suggest an alternative interpretation of the double-labelled cells used by Kintner & Brockes (1984) as evidence for myofibre dedifferentiation in limb regeneration. Instead, we propose that such cells represent new myogenesis occurring by tissue repair of locally damaged muscle fibres.


This article has been cited by other articles:


Home page
 DevelopmentHome page
A. Glavic, F. Silva, M. J. Aybar, F. Bastidas, and R. Mayor
Interplay between Notch signaling and the homeoprotein Xiro1 is required for neural crest induction in Xenopus embryos
Development, January 15, 2004; 131(2): 347 - 359.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
J. Corcoran and P Ferretti
RA regulation of keratin expression and myogenesis suggests different ways of regenerating muscle in adult amphibian limbs
J. Cell Sci., January 5, 1999; 112(9): 1385 - 1394.
[Abstract] [PDF]

Home page
 DevelopmentHome page
S. Devoto, E Melancon, J. Eisen, and M Westerfield
Identification of separate slow and fast muscle precursor cells in vivo, prior to somite formation
Development, January 11, 1996; 122(11): 3371 - 3380.
[Abstract] [PDF]


© The Company of Biologists Ltd 1987