|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
Development, Vol 104, Issue 2 275-284, Copyright © 1988 by Company of Biologists
JOURNAL ARTICLES |
SJ McRobbie, KA Jermyn, K Duffy, K Blight and JG Williams
Imperial Cancer Research Fund, Clare Hall Laboratories, South Mimms, Herts, UK.
The migratory slug of Dictyostelium discoideum is surrounded by, and continuously synthesizes, an extracellular protein-cellulose matrix known as the slime sheath which is deposited on the substratum as a trail marking the slug's progress. We show that the stalk-specific proteins, ST310 and ST430, are exclusively located in the slime sheath and trail and that fusion genes, containing upstream sequences from the cognate genes, direct correct mRNA accumulation during development and correct localization of the fusion protein. Immunoelectron microscopy shows the ST310 and ST430 proteins to be present throughout the entire thickness of the slime sheath and almost totally absent from the cells of the slug. The genes that encode the ST310 and ST430 polypeptides are inducible by DIF, a stalk-specific inducing agent, and the mRNAs are highly enriched in prestalk over prespore cells. The production of these extracellular proteins by prestalk cells suggests that, in a manner somewhat analogous to that of extracellular matrix proteins of higher eukaryotes, the anterior region of the slug may be responsible for the continuous deposition of a track, along which the slug cells migrate. In the mature culminant, the ST310, and possibly the ST430, protein form part of the stalk tube and stalk cell wall. Therefore, the results also show that there are proteins common to both slime trial and stalk tube, indicating a possible precursor-product relationship between these chemically similar integuments.
This article has been cited by other articles:
|
|
 |
|
  T. R. Varney, H. Ho, C. Petty, and D. D. Blumberg A novel disintegrin domain protein affects early cell type specification and pattern formation in Dictyostelium Development, March 7, 2003; 129(10): 2381 - 2389. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Li, C. Foote, S. Alexander, and H. Alexander Sphingosine-1-phosphate lyase has a central role in the development of Dictyostelium discoideum Development, September 15, 2001; 128(18): 3473 - 3483. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. McGuire and S. Alexander PsB Multiprotein Complex of Dictyostelium discoideum. DEMONSTRATION OF CELLULOSE BINDING ACTIVITY AND ORDER OF PROTEIN SUBUNIT ASSEMBLY J. Biol. Chem., June 14, 1996; 271(24): 14596 - 14603. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K Jermyn, D Traynor, and J Williams The initiation of basal disc formation in Dictyostelium discoideum is an early event in culmination Development, January 3, 1996; 122(3): 753 - 760. [Abstract] [PDF]
|
|
|
|
|
|
A. Early, M. Gaskell, D Traynor, and J. Williams Two distinct populations of prestalk cells within the tip of the migratory Dictyostelium slug with differing fates at culmination Development, January 6, 1993; 118(2): 353 - 362. [Abstract] [PDF]
|
|
