|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
Development, Vol 105, Issue 3 505-519, Copyright © 1989 by Company of Biologists
JOURNAL ARTICLES |
PC Letourneau and TA Shattuck
Department of Cell Biology and Neuroanatomy, University of Minnesota, Minneapolis 55455.
Actin filaments and their interactions with cell surface molecules have key roles in tissue cell behaviour. Axonal pathfinding during embryogenesis, an especially complex cell behaviour, is based on the migration of nerve growth cones. We have used fluorescence immunocytochemistry to examine the distribution in growth cones, their filopodia and lamellipodia of several actin-associated proteins and nerve cell adhesion molecules. The leading margins of chick dorsal root ganglion nerve growth cones and their protrusions stain strongly for f-actin, filamin, alpha-actinin, myosin, tropomyosin, talin and vinculin. MAP2 is absent from DRG growth cones, and staining for spectrin fodrin extends into growth cones, but not along filopodia. Thus, organization of the leading margins of growth cones may strongly resemble the leading lamella of migrating fibroblasts. The adhesion-mediating molecules integrin, L1, N-CAM and A-CAM are all found on DRG neurites and growth cones. However, filopodia stain relatively more strongly for integrin and L1 than for A-CAM or N-CAM. In fact, the 180 X 10(3) Mr form of N-CAM may be absent from most of the length of filopodia. DRG neurones cultured in cytochalasin B display differences in immunofluorescence staining which further emphasize that these adhesion molecules interact differentially with the actin filament system of migrating growth cones. Several models for neuronal morphogenesis emphasize the importance of regulation of the expression of adhesion molecules. Our results support hypotheses that cellular distribution and transmembrane interactions are key elements in the functions of these adhesion molecules during axonal pathfinding.
This article has been cited by other articles:
|
|
 |
|
  S. L. Gupton and F. B. Gertler Filopodia: The Fingers That Do the Walking Sci. Signal., August 21, 2007; 2007(400): re5 - re5. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Lovell and L. L. Moroz The largest growth cones in the animal kingdom: an illustrated guide to the dynamics of Aplysia neuronal growth in cell culture Integr. Comp. Biol., December 1, 2006; 46(6): 847 - 870. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. C. Gatlin, A. Estrada-Bernal, S. D. Sanford, and K. H. Pfenninger Myristoylated, Alanine-rich C-Kinase Substrate Phosphorylation Regulates Growth Cone Adhesion and Pathfinding Mol. Biol. Cell, December 1, 2006; 17(12): 5115 - 5130. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Willis, K. W. Li, J.-Q. Zheng, J. H. Chang, A. Smit, T. Kelly, T. T. Merianda, J. Sylvester, J. van Minnen, and J. L. Twiss Differential Transport and Local Translation of Cytoskeletal, Injury-Response, and Neurodegeneration Protein mRNAs in Axons J. Neurosci., January 26, 2005; 25(4): 778 - 791. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Gallo, H. F. Yee Jr., and P. C. Letourneau Actin turnover is required to prevent axon retraction driven by endogenous actomyosin contractility J. Cell Biol., September 29, 2002; 158(7): 1219 - 1228. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. B. Steketee and K. W. Tosney Three Functionally Distinct Adhesions in Filopodia: Shaft Adhesions Control Lamellar Extension J. Neurosci., September 15, 2002; 22(18): 8071 - 8083. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. A. Smith, S. P. Gross, and L. W. Enquist Herpesviruses use bidirectional fast-axonal transport to spread in sensory neurons PNAS, March 13, 2001; 98(6): 3466 - 3470. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. M. Gomez, E. Robles, M.-m. Poo, and N. C. Spitzer Filopodial Calcium Transients Promote Substrate-Dependent Growth Cone Turning Science, March 9, 2001; 291(5510): 1983 - 1987. [Abstract] [Full Text]
|
|
|
|
|
|
G. Pigino, A. Pelsman, H. Mori, and J. Busciglio Presenilin-1 Mutations Reduce Cytoskeletal Association, Deregulate Neurite Growth, and Potentiate Neuronal Dystrophy and Tau Phosphorylation J. Neurosci., February 1, 2001; 21(3): 834 - 842. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Takei, T. A. Chan, F.-S. Wang, H. Deng, U. Rutishauser, and D. G. Jay The Neural Cell Adhesion Molecules L1 and NCAM-180 Act in Different Steps of Neurite Outgrowth J. Neurosci., November 1, 1999; 19(21): 9469 - 9479. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. A. Welnhofer, L. Zhao, and C. S. Cohan Calcium Influx Alters Actin Bundle Dynamics and Retrograde Flow in Helisoma Growth Cones J. Neurosci., September 15, 1999; 19(18): 7971 - 7982. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Francis, R. Ramakrishna, W. Holloway, and B. O. Palsson Two New Pseudopod Morphologies Displayed by the Human Hematopoietic KG1a Progenitor Cell Line and by Primary Human CD34+ Cells Blood, November 15, 1998; 92(10): 3616 - 3623. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Kamiguchi and V. Lemmon A Neuronal Form of the Cell Adhesion Molecule L1 Contains a Tyrosine-Based Signal Required for Sorting to the Axonal Growth Cone J. Neurosci., May 15, 1998; 18(10): 3749 - 3756. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Nakanishi, H. Obaishi, A. Satoh, M. Wada, K. Mandai, K. Satoh, H. Nishioka, Y. Matsuura, A. Mizoguchi, and Y. Takai Neurabin: A Novel Neural Tissue-specific Actin Filament-binding Protein Involved in Neurite Formation J. Cell Biol., November 17, 1997; 139(4): 951 - 961. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Furukawa, W. Fu, Y. Li, W. Witke, D. J. Kwiatkowski, and M. P. Mattson The Actin-Severing Protein Gelsolin Modulates Calcium Channel and NMDA Receptor Activities and Vulnerability to Excitotoxicity in Hippocampal Neurons J. Neurosci., November 1, 1997; 17(21): 8178 - 8186. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Lu, W. Witke, D. J. Kwiatkowski, and K. S. Kosik Delayed Retraction of Filopodia in Gelsolin Null Mice J. Cell Biol., September 22, 1997; 138(6): 1279 - 1287. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Gallo, F. B. Lefcort, and P. C. Letourneau The trkA Receptor Mediates Growth Cone Turning toward a Localized Source of Nerve Growth Factor J. Neurosci., July 15, 1997; 17(14): 5445 - 5454. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Mascotti, A. Caceres, K. H. Pfenninger, and S. Quiroga Expression and Distribution of IGF-1 Receptors Containing a beta -Subunit Variant (beta gc) in Developing Neurons J. Neurosci., February 15, 1997; 17(4): 1447 - 1459. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. H. Le Gall, S. K. Powell, C. A. Yeaman, and E. Rodriguez-Boulan The Neural Cell Adhesion Molecule Expresses a Tyrosine-independent Basolateral Sorting Signal J. Biol. Chem., February 14, 1997; 272(7): 4559 - 4567. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. M. Strittmatter Signal Transduction at the Neuronal Growth Cone Neuroscientist, March 1, 1996; 2(2): 83 - 86. [Abstract] [PDF]
|
|
|
|
 |
|
 J. Challacombe, D. Snow, and P. Letourneau Actin filament bundles are required for microtubule reorientation during growth cone turning to avoid an inhibitory guidance cue J. Cell Sci., January 8, 1996; 109(8): 2031 - 2040. [Abstract] [PDF]
|
|
|
|
 |
|
 A. Stoker, B Gehrig, F Haj, and B. Bay Axonal localisation of the CAM-like tyrosine phosphatase CRYP alpha: a signalling molecule of embryonic growth cones Development, January 6, 1995; 121(6): 1833 - 1844. [Abstract] [PDF]
|
|
|
|
|
|
L Had, C Faivre-Sarrailh, C Legrand, J Mery, J Brugidou, and A Rabie Tropomyosin isoforms in rat neurons: the different developmental profiles and distributions of TM-4 and TMBr-3 are consistent with different functions J. Cell Sci., January 10, 1994; 107(10): 2961 - 2973. [Abstract] [PDF]
|
|
