Cytoplasmic regulation of translation during Ilyanassa embryogenesis

Two-dimensional electrophoresis of labeled proteins and hybridization of mRNAs with specific gene probes was demonstrate changes in protein synthesis, and the of actin maternal mRNAs during the early development isolated polar lobe was used as a nucleus-free egg the regulation of translation. It was shown that actin present in the unfertilized egg and are therefore are inactive during early cleavage, and are translated lobeless, actinomycin D-treated blastulae and in that have been aged for 24h. Thus, the activation of controlled by cytoplasmic factors that function cleavage and nuclear activity. I suggest that the cytoplasmic clock determines when maternal mRNAs are that this clock is made and set running during protein synthesis that occurred during early cleavage also involve the diminution of some early cleavage suggested that this diminution is controlled by localized in the blastomeres of the lobeless egg but polar lobe.