spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Heath, J. K.
Right arrow Articles by Edwards, D. R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Heath, J. K.
Right arrow Articles by Edwards, D. R.

Development, Vol 107, Issue 1 113-122, Copyright © 1989 by Company of Biologists

JOURNAL ARTICLES

Expression of multiple heparin-binding growth factor species by murine embryonal carcinoma and embryonic stem cells

JK Heath, GD Paterno, AC Lindon and DR Edwards
Department of Biochemistry, University of Oxford, UK.

Culture medium conditioned by P19 embryonal carcinoma (EC) cells contains potent mitogenic activity which is markedly potentiated when the medium is conditioned in the presence of heparin. Fractionation of P19 medium conditioned in the presence of heparin reveals the existence of two biochemically distinct growth factor species both of which exhibit high affinity for immobilised heparin and significant activity as amphibian mesoderm-inducing agents. One of the species is recovered as a single polypeptide of apparent Mr = 15,000. This molecule is immunologically related to the protein product of the human K-FGF proto-oncogene. Transcripts derived from the murine K-FGF gene are also expressed by both differentiated and undifferentiated EC cells and embryonic stem cells. The second heparin-binding growth factor is recovered as a complex of four polypeptides, the largest of which has an apparent Mr = 17,000. This agent is immunologically and biochemically distinct from both acidic and basic fibroblast growth factor as well as K-FGF, and represents the predominant mitogenic activity in EC-cell-conditioned medium.


This article has been cited by other articles:


Home page
 JCBHome page
Y. Mao and A. W.-M. Lee
A novel role for Gab2 in bFGF-mediated cell survival during retinoic acid-induced neuronal differentiation
J. Cell Biol., July 18, 2005; 170(2): 305 - 316.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
V. Nurcombe, N. Fraser, E. Herlaar, and J. K. Heath
MK: a pluripotential embryonic stem-cell-derived neuroregulatory factor
Development, December 1, 1992; 116(4): 1175 - 1183.
[Abstract] [PDF]


© The Company of Biologists Ltd 1989