spacer gif spacer gif spacer gif spacer gif ARCHIVE ANNOUNCEMENT! spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Rudnicki, M. A.
Right arrow Articles by McBurney, M. W.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Rudnicki, M. A.
Right arrow Articles by McBurney, M. W.

Development, Vol 107, Issue 2 361-372, Copyright © 1989 by Company of Biologists

JOURNAL ARTICLES

Cell lines with developmental potential restricted to mesodermal lineages isolated from differentiating cultures of pluripotential P19 embryonal carcinoma cells

MA Rudnicki, KR Reuhl and MW McBurney
Department of Medicine, University of Ottawa, Ontario, Canada.

Embryonal carcinoma (EC) cells are developmentally pluripotential cells which can be induced to differentiate in cell culture to form a wide variety of cell types. To investigate the lineage relationships between cells of different types, we set out to isolate cell lines with multiple but restricted developmental potentials from differentiating cultures of P19 cells, a line of EC. By selecting for differentiated cells capable of anchorage-independent growth, we isolated cell lines which differentiated in high density cultures to form at least two cell types; myocytes that resembled fetal skeletal muscle cells and loose connective tissue cells that secreted large amounts of type I collagen. These results suggest that skeletal myocytes and connective tissue share a common precursor and that stem cells with limited but multiple developmental potentials can be isolated from differentiating cultures of P19 cells.


This article has been cited by other articles:


Home page
 Cardiovasc ResHome page
M. A.G. van der Heyden and L. H.K. Defize
Twenty one years of P19 cells: what an embryonal carcinoma cell line taught us about cardiomyocyte differentiation
Cardiovasc Res, May 1, 2003; 58(2): 292 - 302.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
C. Menard, S. Pupier, D. Mornet, M. Kitzmann, J. Nargeot, and P. Lory
Modulation of L-type Calcium Channel Expression during Retinoic Acid-induced Differentiation of H9C2 Cardiac Cells
J. Biol. Chem., October 8, 1999; 274(41): 29063 - 29070.
[Abstract] [Full Text] [PDF]

Home page
 Circ. Res.Home page
M. C. Colbert, M. L. Kirby, and J. Robbins
Endogenous Retinoic Acid Signaling Colocalizes With Advanced Expression of the Adult Smooth Muscle Myosin Heavy Chain Isoform During Development of the Ductus Arteriosus
Circ. Res., May 1, 1996; 78(5): 790 - 798.
[Abstract] [Full Text]

Home page
 Circ. Res.Home page
R. S. Blank, E. A. Swartz, M. M. Thompson, E. N. Olson, and G. K. Owens
A Retinoic Acid–Induced Clonal Cell Line Derived From Multipotential P19 Embryonal Carcinoma Cells Expresses Smooth Muscle Characteristics
Circ. Res., May 1, 1995; 76(5): 742 - 749.
[Abstract] [Full Text]


© The Company of Biologists Ltd 1989