|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
Development, Vol 107, Issue 4 891-897, Copyright © 1989 by Company of Biologists
JOURNAL ARTICLES |
M Wilcox, A DiAntonio and M Leptin
MRC Laboratory of Molecular Biology, Cambridge, UK.
Integrins are found on many cell types during the development of most organisms. In Drosophila their functions can be analysed genetically. An analysis of lethal mutations in a PS integrin gene showed that the integrins were required for muscle attachment and for certain cell sheet migrations during embryogenesis. In this paper we use viable mutations in integrin component genes to look at integrin function in the later stages of development of one adult structure, the wing. We show that two known viable mutations, one which has its primary effect on the fly's escape response, the other on wing morphogenesis, are mutations in the beta and PS2alpha subunits, respectively, of the PS integrins. The mutation non-jumper (mys(mj42)) in the beta subunit leads to wasting of the thoracic jump muscles. Flies in which the dosage of this allele is reduced (and no wildtype copy is present) show defects also in wing morphogenesis. The two surfaces of the wing fail to connect properly, resulting in 'blistering' of the wing and the formation of extra crossveins. The mutation in the gene for the PS2alpha integrin subunit, inflated, also leads to a failure in wing surface apposition and consequent wing blistering. When the two mutations are combined, the mutant phenotype is greatly enhanced. Thus, one of the roles of the PS integrins in late Drosophila development is to ensure the correct apposition and patterning of the wing epithelia.
This article has been cited by other articles:
|
|
 |
|
  S. Murakami, D. Umetsu, Y. Maeyama, M. Sato, S. Yoshida, and T. Tabata Focal adhesion kinase controls morphogenesis of the Drosophila optic stalk Development, April 15, 2007; 134(8): 1539 - 1548. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Dominguez-Gimenez, N. H. Brown, and M. D. Martin-Bermudo Integrin-ECM interactions regulate the changes in cell shape driving the morphogenesis of the Drosophila wing epithelium J. Cell Sci., March 15, 2007; 120(6): 1061 - 1071. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Grabbe, C. G. Zervas, T. Hunter, N. H. Brown, and R. H. Palmer Focal adhesion kinase is not required for integrin function or viability in Drosophila Development, December 1, 2004; 131(23): 5795 - 5805. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. B. Lee, K. S. Cho, E. Kim, and J. Chung blistery encodes Drosophila tensin protein and interacts with integrin and the JNK signaling pathway during wing development Development, September 1, 2003; 130(17): 4001 - 4010. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Araujo, E. Negreiros, and E. Bier Integrins modulate Sog activity in the Drosophila wing Development, August 15, 2003; 130(16): 3851 - 3864. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. E. Baker, J. A. Lorenzen, S. W. Miller, T. A. Bunch, A. L. Jannuzi, M. H. Ginsberg, L. A. Perkins, and D. L. Brower Genetic Interaction Between Integrins and moleskin, a Gene Encoding a Drosophila Homolog of Importin-7 Genetics, September 1, 2002; 162(1): 285 - 296. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. H. Palmer, L. I. Fessler, P. T. Edeen, S. J. Madigan, M. McKeown, and T. Hunter DFak56 Is a Novel Drosophila melanogaster Focal Adhesion Kinase J. Biol. Chem., December 10, 1999; 274(50): 35621 - 35629. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. E. Stronach, P. J. Renfranz, B. Lilly, and M. C. Beckerle Muscle LIM Proteins Are Associated with Muscle Sarcomeres and Require dMEF2 for Their Expression during Drosophila Myogenesis Mol. Biol. Cell, July 1, 1999; 10(7): 2329 - 2342. [Abstract] [Full Text]
|
|
|
|
 |
|
 B. Hoang and A. Chiba Genetic Analysis on the Role of Integrin during Axon Guidance in Drosophila J. Neurosci., October 1, 1998; 18(19): 7847 - 7855. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. P. Walsh and N. H. Brown A Screen to Identify Drosophila Genes Required for Integrin-Mediated Adhesion Genetics, October 1, 1998; 150(2): 791 - 805. [Abstract] [Full Text]
|
|
|
|
|
|
J. W. Bloor and N. H. Brown Genetic Analysis of the Drosophila {alpha}PS2 Integrin Subunit Reveals Discrete Adhesive, Morphogenetic and Sarcomeric Functions Genetics, March 1, 1998; 148(3): 1127 - 1142. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X Li, M. Graner, E. Williams, C. Roote, T. Bunch, and S Zusman Requirements for the cytoplasmic domain of the alphaPS1, alphaPS2 and betaPS integrin subunits during Drosophila development Development, January 2, 1998; 125(4): 701 - 711. [Abstract] [PDF]
|
|
|
|
 |
|
 J R Riesgo-Escovar and E Hafen Drosophila Jun kinase regulates expression of decapentaplegic via the ETS-domain protein Aop and the AP-1 transcription factor DJun during dorsal closure. Genes & Dev., July 1, 1997; 11(13): 1717 - 1727. [Abstract] [PDF]
|
|
|
|
|
|
X S Hou, E S Goldstein, and N Perrimon Drosophila Jun relays the Jun amino-terminal kinase signal transduction pathway to the Decapentaplegic signal transduction pathway in regulating epithelial cell sheet movement. Genes & Dev., July 1, 1997; 11(13): 1728 - 1737. [Abstract] [PDF]
|
|
|
|
|
|
K. Stark, G. Yee, C. Roote, E. Williams, S Zusman, and R. Hynes A novel alpha integrin subunit associates with betaPS and functions in tissue morphogenesis and movement during Drosophila development Development, January 11, 1997; 124(22): 4583 - 4594. [Abstract] [PDF]
|
|
|
|
|
|
J R Riesgo-Escovar, M Jenni, A Fritz, and E Hafen The Drosophila Jun-N-terminal kinase is required for cell morphogenesis but not for DJun-dependent cell fate specification in the eye. Genes & Dev., November 1, 1996; 10(21): 2759 - 2768. [Abstract] [PDF]
|
|
|
|
|
|
M. Brabant, D Fristrom, T. Bunch, and D. Brower Distinct spatial and temporal functions for PS integrins during Drosophila wing morphogenesis Development, January 10, 1996; 122(10): 3307 - 3317. [Abstract] [PDF]
|
|
|
|
|
|
J Montagne, J Groppe, K Guillemin, M. Krasnow, W. Gehring, and M Affolter The Drosophila Serum Response Factor gene is required for the formation of intervein tissue of the wing and is allelic to blistered Development, January 9, 1996; 122(9): 2589 - 2597. [Abstract] [PDF]
|
|
|
|
|
|
C. Roote and S Zusman Alternatively spliced forms of the Drosophila alphaPS2 subunit of integrin are sufficient for viability and can replace the function of the alphaPS1 subunit of integrin in the retina Development, January 6, 1996; 122(6): 1985 - 1994. [Abstract] [PDF]
|
|
|
|
|
|
B Murugasu-Oei, V Rodrigues, X Yang, and W Chia Masquerade: a novel secreted serine protease-like molecule is required for somatic muscle attachment in the Drosophila embryo. Genes & Dev., January 15, 1995; 9(2): 139 - 154. [Abstract] [PDF]
|
|
|
|
|
|
M. Pankratz and M Hoch Control of epithelial morphogenesis by cell signaling and integrin molecules in the Drosophila foregut Development, January 6, 1995; 121(6): 1885 - 1898. [Abstract] [PDF]
|
|
|
|
|
|
D. Brower, T. Bunch, L Mukai, T. Adamson, M Wehrli, S Lam, E Friedlander, C. Roote, and S Zusman Nonequivalent requirements for PS1 and PS2 integrin at cell attachments in Drosophila: genetic analysis of the alpha PS1 integrin subunit Development, January 5, 1995; 121(5): 1311 - 1320. [Abstract] [PDF]
|
|
|
|
|
|
M. Sturtevant and E Bier Analysis of the genetic hierarchy guiding wing vein development in Drosophila Development, January 3, 1995; 121(3): 785 - 801. [Abstract] [PDF]
|
|
|
|
|
|
D. Fristrom, P. Gotwals, S. Eaton, T. B. Kornberg, M. Sturtevant, E. Bier, and J. W. Fristrom Blistered: a gene required for vein/intervein formation in wings of Drosophila Development, September 1, 1994; 120(9): 2661 - 2671. [Abstract] [PDF]
|
|
|
|
|
|
F. Fogerty, L. Fessler, T. Bunch, Y Yaron, C. Parker, R. Nelson, D. Brower, D Gullberg, and J. Fessler Tiggrin, a novel Drosophila extracellular matrix protein that functions as a ligand for Drosophila alpha PS2 beta PS integrins Development, January 7, 1994; 120(7): 1747 - 1758. [Abstract] [PDF]
|
|
|
|
|
|
S. Blair, D. Brower, J. Thomas, and M Zavortink The role of apterous in the control of dorsoventral compartmentalization and PS integrin gene expression in the developing wing of Drosophila Development, January 7, 1994; 120(7): 1805 - 1815. [Abstract] [PDF]
|
|
|
|
|
|
N. Brown Null mutations in the alpha PS2 and beta PS integrin subunit genes have distinct phenotypes Development, January 5, 1994; 120(5): 1221 - 1231. [Abstract] [PDF]
|
|
|
|
|
|
Y Grinblat, S Zusman, G Yee, R. Hynes, and F. Kafatos Functions of the cytoplasmic domain of the beta PS integrin subunit during Drosophila development Development, January 1, 1994; 120(1): 91 - 102. [Abstract] [PDF]
|
|
|
|
|
|
M A Sturtevant, M Roark, and E Bier The Drosophila rhomboid gene mediates the localized formation of wing veins and interacts genetically with components of the EGF-R signaling pathway. Genes & Dev., June 1, 1993; 7(6): 961 - 973. [Abstract] [PDF]
|
|
|
|
|
|
S Zusman, Y Grinblat, G Yee, F. Kafatos, and R. Hynes Analyses of PS integrin functions during Drosophila development Development, January 7, 1993; 118(3): 737 - 750. [Abstract] [PDF]
|
|
|
|
|
|
G. Yee and R. Hynes A novel, tissue-specific integrin subunit, beta nu, expressed in the midgut of Drosophila melanogaster Development, January 7, 1993; 118(3): 845 - 858. [Abstract] [PDF]
|
|
|
|
|
|
J. Adams and F. Watt Regulation of development and differentiation by the extracellular matrix Development, January 4, 1993; 117(4): 1183 - 1198. [PDF]
|
|
|
|
|
|
D Fristrom, M Wilcox, and J Fristrom The distribution of PS integrins, laminin A and F-actin during key stages in Drosophila wing development Development, January 2, 1993; 117(2): 509 - 523. [Abstract] [PDF]
|
|
|
|
|
|
T. Bunch and D. Brower Drosophila PS2 integrin mediates RGD-dependent cell-matrix interactions Development, January 9, 1992; 116(1): 239 - 247. [Abstract] [PDF]
|
|
