spacer gif spacer gif spacer gif spacer gif ARCHIVE ANNOUNCEMENT! spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Yu, C. C.
Right arrow Articles by Breitman, M. L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Yu, C. C.
Right arrow Articles by Breitman, M. L.

Development, Vol 110, Issue 1 131-139, Copyright © 1990 by Company of Biologists

JOURNAL ARTICLES

Homologous and heterologous enhancers modulate spatial expression but not cell-type specificity of the murine gamma F-crystallin promoter

CC Yu, LC Tsui and ML Breitman
Division of Molecular and Developmental Biology, Mount Sinai Hospital, Toronto, Ontario, Canada.

Previous studies have shown that mouse gamma F-crystallin sequences -759 to +45, which include the core promoter and two upstream enhancer elements, contain sufficient information for directing gene expression to terminally differentiated fiber cells of the ocular lens. To investigate the role that proximal sequences of the mouse gamma F-crystallin promoter play in the developmental regulation of gene expression, we generated transgenic mice containing the lacZ gene driven by either mouse gamma F-crystallin sequences -171 to +45, which lack functional enhancers, or a hybrid hamster alpha A-/mouse gamma F-crystallin promoter, which contains the hamster alpha A-crystallin enhancer instead of operational gamma F-crystallin enhancers. In situ analysis of lacZ expression in these mice revealed that the mouse gamma F-crystallin promoter segment -171 to +45, which shows low activity in vitro, is able to direct gene expression to the fiber cells in the nucleus of the lens. However, animals expressing gamma 171-lacZ show both a lower level of expression of the lacZ gene and a narrower pattern of staining in the lens nucleus than mice expressing gamma 759-lacZ, which contains the two enhancer elements located between -392 and -278 and -226 to -123.(ABSTRACT TRUNCATED AT 250 WORDS)


This article has been cited by other articles:


Home page
 IOVSHome page
H. Zhao, Y. Yang, C. M. Rizo, P. A. Overbeek, and M. L. Robinson
Insertion of a Pax6 Consensus Binding Site into the {alpha}A-Crystallin Promoter Acts as a Lens Epithelial Cell Enhancer in Transgenic Mice
Invest. Ophthalmol. Vis. Sci., June 1, 2004; 45(6): 1930 - 1939.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
E. J. Klok, S. T. van Genesen, A. Civil, J. G. G. Schoenmakers, and N. H. Lubsen
Regulation of Expression within a Gene Family. THE CASE OF THE RAT gamma B- AND gamma D-CRYSTALLIN PROMOTERS
J. Biol. Chem., July 3, 1998; 273(27): 17206 - 17215.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
X. Wang, S. Zinkel, K. Polonsky, and E. Fuchs
Transgenic studies with a keratin promoter-driven growth hormone transgene: Prospects for gene therapy
PNAS, January 7, 1997; 94(1): 219 - 226.
[Abstract] [Full Text] [PDF]

Home page
 Genes Dev.Home page
M Tini, G Otulakowski, M L Breitman, L C Tsui, and V Giguere
An everted repeat mediates retinoic acid induction of the gamma F-crystallin gene: evidence of a direct role for retinoids in lens development.
Genes & Dev., February 1, 1993; 7(2): 295 - 307.
[Abstract] [PDF]


© The Company of Biologists Ltd 1990