spacer gif spacer gif spacer gif spacer gif ARCHIVE ANNOUNCEMENT! spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Thompson, M.
Right arrow Articles by Wright, N. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Thompson, M.
Right arrow Articles by Wright, N. A.

Development, Vol 110, Issue 2 477-481, Copyright © 1990 by Company of Biologists

JOURNAL ARTICLES

Gastric endocrine cells share a clonal origin with other gut cell lineages

M Thompson, KA Fleming, DJ Evans, R Fundele, MA Surani and NA Wright
Department of Histopathology, Royal Postgraduate Medical School, London, UK.

There has been considerable debate about the ontological origin of gut endocrine cells as being either from the neural crest (or primitive epiblast) or from the endodermal stem cell. We have attempted to define the ontological origin of endocrine cells by applying an experimental system that uses a marker to identify one of the two phenotypes present in chimaeric mice as suggested by Ponder et al. (1985). This study involved two separate experiments. The first made use of the unique staining properties of Dolichos biflorus agglutinin (DBA), a lectin that binds to the N-acetyl galactosamine sugar residues present on the surface of C57Bl mouse gut, but absent from RoRIII mouse gut, in C57Bl----RoIII mouse chimaeras at the ultrastructural level. A four-stage procedure for staining at the EM level was developed. Although mature villous endocrine cells stained for DBA, immature endocrine cells did not, either in the positive crypts of chimaeric mouse gut or in gut from C57Bl positive controls. Thus a second marker was chosen. This experiment combined immunocytochemistry (to identify gastric antral gastrin cells chosen as a representative neuroendocrine cell) with in situ DNA hybridization for the mouse male chromosome repeat sequence PY 353 (to identify XY cells) in XX----XY chimaeric mice. This study showed that the sex chromosomal pattern in the gastrin cells parallels that of other cells in the same gastric gland and therefore are clonal with them. This suggests that gut endocrine cells share a common stem cell with other epithelial cell lineages in the antrum and are endodermally derived.


This article has been cited by other articles:


Home page
 J. Exp. Biol.Home page
I. Illa-Bochaca and L. M. Montuenga
The regenerative nidi of the locust midgut as a model to study epithelial cell differentiation from stem cells
J. Exp. Biol., June 1, 2006; 209(11): 2215 - 2223.
[Abstract] [Full Text] [PDF]

Home page
 GutHome page
M Brittan and N A Wright
STEM CELL IN GASTROINTESTINAL STRUCTURE AND NEOPLASTIC DEVELOPMENT
Gut, June 1, 2004; 53(6): 899 - 910.
[Full Text] [PDF]

Home page
 DevelopmentHome page
J. Aubin, U. Dery, M. Lemieux, P. Chailler, and L. Jeannotte
Stomach regional specification requires Hoxa5-driven mesenchymal-epithelial signaling
Development, September 1, 2002; 129(17): 4075 - 4087.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
M. Bjerknes and H. Cheng
Multipotential stem cells in adult mouse gastric epithelium
Am J Physiol Gastrointest Liver Physiol, September 1, 2002; 283(3): G767 - G777.
[Abstract] [Full Text] [PDF]


© The Company of Biologists Ltd 1990