|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
Development, Vol 110, Issue 4 1285-1293, Copyright © 1990 by Company of Biologists
JOURNAL ARTICLES |
A Kucherer-Ehret, J Pottgiesser, GW Kreutzberg, H Thoenen and D Edgar
Max Planck Institute for Psychiatry, Martinsried, Federal Republic of Germany.
The expression of the polypeptide subunits of the glycoprotein laminin in developing mouse tissues was analysed by immunoblots and Northern blots, and by immunohistochemistry at the ultrastructural level. In the neonate, almost all the laminin of the sciatic nerve was freely extractable and was located mainly in the mesenchymal interstitial extracellular matrix, rather than in basement membranes. During the first two postnatal weeks, the distribution of laminin shifted to assume the adult pattern, most being located in basement membranes and insoluble under physiological conditions. Analysis of laminin subunit expression showed that both the mRNA for the laminin B chains and the corresponding polypeptides are widely expressed in nerve and other tissues, the mRNA levels decreasing during the first two postnatal weeks as the amount of laminin in the tissue increased. In contrast, the A chain mRNA and polypeptide were undetectable in nerve at any age studied, although they were present in perinatal kidney and placenta. It is proposed that the large amount of soluble laminin present in the developing interstitial extracellular matrix is a consequence of the high levels of expression of laminin mRNA, the subsequent decrease in expression resulting in the adult distribution where most laminin is insoluble within the basement membrane.
This article has been cited by other articles:
|
|
 |
|
  B. L. Bader, N. Smyth, S. Nedbal, N. Miosge, A. Baranowsky, S. Mokkapati, M. Murshed, and R. Nischt Compound Genetic Ablation of Nidogen 1 and 2 Causes Basement Membrane Defects and Perinatal Lethality in Mice Mol. Cell. Biol., August 1, 2005; 25(15): 6846 - 6856. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P Murray and D Edgar Regulation of the differentiation and behaviour of extra-embryonic endodermal cells by basement membranes J. Cell Sci., January 3, 2001; 114(5): 931 - 939. [Abstract] [PDF]
|
|
|
|
|
|
M. Murshed, N. Smyth, N. Miosge, J. Karolat, T. Krieg, M. Paulsson, and R. Nischt The Absence of Nidogen 1 Does Not Affect Murine Basement Membrane Formation Mol. Cell. Biol., September 15, 2000; 20(18): 7007 - 7012. [Abstract] [Full Text]
|
|
|
|
 |
|
 P. Murray and D. Edgar Regulation of Programmed Cell Death by Basement Membranes in Embryonic Development J. Cell Biol., September 5, 2000; 150(5): 1215 - 1221. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Smyth, H. S. Vatansever, P. Murray, M. Meyer, C. Frie, M. Paulsson, and D. Edgar Absence of Basement Membranes after Targeting the LAMC1 Gene Results in Embryonic Lethality Due to Failure of Endoderm Differentiation J. Cell Biol., January 11, 1999; 144(1): 151 - 160. [Abstract] [Full Text] [PDF]
|
|
