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Development, Vol 111, Issue 1 221-232, Copyright © 1991 by Company of Biologists
JOURNAL ARTICLES |
E Bloch-Gallego, M Huchet, H el M'Hamdi, FK Xie, H Tanaka and CE Henderson
UPR 8402 du CNRS-U 249 de l'INSERM, Montpellier, France.
Motoneurons were identified in vitro by a new method using the SC1 monoclonal antibody. They constituted up to 30% of total neurons in cultures of whole spinal cord from 4.5-day chicken embryos, and survived for at least 5 days in the presence of muscle extract, but not in its absence. By contrast, other neurons and floor-plate cells survived without muscle-derived factors. Motoneurons were purified to homogeneity by 'panning' on dishes coated with SC1 antibody; they developed rapidly even in the absence of other spinal cells. Concentrations of muscle extract required for half-maximal motoneuron survival were indistinguishable in pure and mixed cultures, suggesting that muscle-derived factors act directly on motoneurons. Other purified growth factors tested, including ciliary neurotrophic factor, did not have the survival-promoting activity of muscle.
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