|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
Development, Vol 111, Issue 2 489-496, Copyright © 1991 by Company of Biologists
JOURNAL ARTICLES |
R Brown and JP Brockes
Ludwig Institute for Cancer Research, Middlesex Hospital/University College Branch, London, UK.
Adult urodele amphibians are able to regenerate their limbs through the formation of a blastema, a growth zone of undifferentiated mesenchymal cells that arises locally at the plane of amputation. In an effort to define genes involved in pattern formation by the blastema, we isolated from a newt forelimb blastema library cDNA clones that identify a homeobox gene termed NvHbox 2. The amino acid sequence of the homeodomain is identical to that of the recently identified human HOX-4f gene (Acampora et al. 1989) and of the mouse Hox-5.5 (Dolle et al. 1989). NvHbox 2 is expressed in the limb blastema as a transcript of 3.4 kb that is not detectable in the normal limb. Analysis by RNAase protection demonstrates expression in limb and tail blastemas, but not in any of the adult tissues or organs tested. In the limb blastema NvHbox 2 was expressed in mesenchymal but not epithelial tissue. When matched and normalised samples of RNA from proximal (mid-humerus) and distal forelimb (mid-radius) blastemas were compared, the level of expression of NvHbox 2 was found to be 3- to 5-fold higher proximally. At two time points after injection of a proximalising dose of retinoic acid, the level of expression in a distal blastema was not increased in comparison to controls.
This article has been cited by other articles:
|
|
 |
|
  N. Mercader, E. M. Tanaka, and M. Torres Proximodistal identity during vertebrate limb regeneration is regulated by Meis homeodomain proteins Development, September 15, 2005; 132(18): 4131 - 4142. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Corcoran and P Ferretti RA regulation of keratin expression and myogenesis suggests different ways of regenerating muscle in adult amphibian limbs J. Cell Sci., January 5, 1999; 112(9): 1385 - 1394. [Abstract] [PDF]
|
|
|
|
 |
|
 Y. Imokawa and K. Yoshizato Expression of Sonic hedgehog gene in regenerating newt limb blastemas recapitulates that in developing limb buds PNAS, August 19, 1997; 94(17): 9159 - 9164. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. M. Tanaka, A. A.F. Gann, P. B. Gates, and J. P. Brockes Newt Myotubes Reenter the Cell Cycle by Phosphorylation of the Retinoblastoma Protein J. Cell Biol., January 13, 1997; 136(1): 155 - 165. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Simon, R Kittappa, P. Khan, C Tsilfidis, R. Liversage, and S Oppenheimer A novel family of T-box genes in urodele amphibian limb development and regeneration: candidate genes involved in vertebrate forelimb/hindlimb patterning Development, January 4, 1997; 124(7): 1355 - 1366. [Abstract] [PDF]
|
|
|
|
|
|
D. Gardiner, B Blumberg, Y Komine, and S. Bryant Regulation of HoxA expression in developing and regenerating axolotl limbs Development, January 6, 1995; 121(6): 1731 - 1741. [Abstract] [PDF]
|
|
|
|
|
|
M. Akimenko, S. Johnson, M Westerfield, and M Ekker Differential induction of four msx homeobox genes during fin development and regeneration in zebrafish Development, January 2, 1995; 121(2): 347 - 357. [Abstract] [PDF]
|
|
|
|
|
|
W. Brook, L. Ostafichuk, J Piorecky, M. Wilkinson, D. Hodgetts, and M. Russell Gene expression during imaginal disc regeneration detected using enhancer-sensitive P-elements Development, January 4, 1993; 117(4): 1287 - 1297. [Abstract] [PDF]
|
|
|
|
|
|
H. Simon and C. Tabin Analysis of Hox-4.5 and Hox-3.6 expression during newt limb regeneration: differential regulation of paralogous Hox genes suggest different roles for members of different Hox clusters Development, January 4, 1993; 117(4): 1397 - 1407. [Abstract] [PDF]
|
|
|
|
|
|
D. Hill, C. Ragsdale, and J. Brockes Isoform-specific immunological detection of newt retinoic acid receptor delta 1 in normal and regenerating limbs Development, January 3, 1993; 117(3): 937 - 945. [Abstract] [PDF]
|
|
