|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
Development, Vol 111, Issue 2 543-549, Copyright © 1991 by Company of Biologists
JOURNAL ARTICLES |
Y Miwa, T Atsumi, N Imai and Y Ikawa
Laboratory of Molecular Oncology, Tsukuba Life Science Center, Institute of Physical and Chemical Research, Ibaraki, Japan.
Mouse teratocarcinoma stem cells PCC3/A/1 differentiated into various types of cells, such as red cells, when they were grown in serum-free medium containing transferrin and bovine serum albumin on a KCF cell feeder layer. These red cells were stained well with 2,7-diaminofluorene (DAF), and therefore were erythroid cells. They were nucleated and contained embryonic globin chains, immunologically identified with antiembryonic hemoglobin antisera after acid urea Triton X-100 polyacrylamide gel electrophoresis (UT-PAGE). The addition of erythropoietin to the culture medium enhanced the production of both embryonic and adult globin chains. The addition of interleukin-3 also enhanced the production of embryonic globin chains, but not the production of adult globin chains. These results indicated that primitive erythropoiesis of PCC3/A/1 teratocarcinoma cells did not require exogenous addition of any hematopoietic factor such as erythropoietin or interleukin-3. This culture system will be a new model system for investigating the factors regulating the primitive erythropoiesis in yolk sac blood islands.
This article has been cited by other articles:
|
|
 |
|
  T. Ueda, R. Watanabe-Fukunaga, H. Ogawa, H. Fukuyama, Y. Higashi, S. Nagata, and R. Fukunaga Critical role of the p400/mDomino chromatin-remodeling ATPase in embryonic hematopoiesis Genes Cells, May 1, 2007; 12(5): 581 - 592. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W.-L. Li, Y. Yamada, M. Ueno, S. Nishikawa, S.-I. Nishikawa, and N. Takakura Platelet Derived Growth Factor Receptor Alpha Is Essential for Establishing a Microenvironment That Supports Definitive Erythropoiesis J. Biochem., August 1, 2006; 140(2): 267 - 273. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Sugiyama, M. Ogawa, I. Hirose, T. Jaffredo, K.-i. Arai, and K. Tsuji Erythropoiesis from acetyl LDL incorporating endothelial cells at the preliver stage Blood, June 15, 2003; 101(12): 4733 - 4738. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Harigae, O. Nakajima, N. Suwabe, H. Yokoyama, K. Furuyama, T. Sasaki, M. Kaku, M. Yamamoto, and S. Sassa Aberrant iron accumulation and oxidized status of erythroid-specific delta -aminolevulinate synthase (ALAS2)-deficient definitive erythroblasts Blood, February 1, 2003; 101(3): 1188 - 1193. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Nishikawa, S Nishikawa, M Hirashima, N Matsuyoshi, and H Kodama Progressive lineage analysis by cell sorting and culture identifies FLK1+VE-cadherin+ cells at a diverging point of endothelial and hemopoietic lineages Development, January 5, 1998; 125(9): 1747 - 1757. [Abstract] [PDF]
|
|
|
|
 |
|
 S. Takahashi, K. Onodera, H. Motohashi, N. Suwabe, N. Hayashi, N. Yanai, Y. Nabesima, and M. Yamamoto Arrest in Primitive Erythroid Cell Development Caused by Promoter-specific Disruption of the GATA-1 Gene J. Biol. Chem., May 9, 1997; 272(19): 12611 - 12615. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Onodera, S. Takahashi, S. Nishimura, J. Ohta, H. Motohashi, K. Yomogida, N. Hayashi, J. D. Engel, and M. Yamamoto GATA-1 transcription is controlled by distinct regulatory mechanisms during primitive and definitive erythropoiesis PNAS, April 29, 1997; 94(9): 4487 - 4492. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Era, T. Takahashi, K. Sakai, K. Kawamura, and T. Nakano Thrombopoietin Enhances Proliferation and Differentiation of Murine Yolk Sac Erythroid Progenitors Blood, February 15, 1997; 89(4): 1207 - 1213. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Weinstein, A. Schier, S Abdelilah, J Malicki, L Solnica-Krezel, D. Stemple, D. Stainier, F Zwartkruis, W Driever, and M. Fishman Hematopoietic mutations in the zebrafish Development, January 12, 1996; 123(1): 303 - 309. [Abstract] [PDF]
|
|
