|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
Development, Vol 111, Issue 4 1061-1080, Copyright © 1991 by Company of Biologists
JOURNAL ARTICLES |
R Hardy and R Reynolds
Department of Biochemistry, Imperial College of Science, Technology and Medicine, London, UK.
We have followed the development of the O-2A progenitor cell from the neonatal rat forebrain, both in dissociated cell culture and in cryostat sections, using immunocytochemical techniques employing a panel of antibodies that recognise the cells at different stages of their development. This included the monoclonal antibody LB1, which binds to the surface ganglioside GD3 expressed on O-2A progenitor cells. In secondary cultures enriched for O-2A progenitors maintained in a serum-free chemically defined medium, a large proportion of the cells are primed to differentiate into oligodendroglia and go on to express the oligodendroglial specific surface glycolipid galactocerebroside (GC) and then the myelin proteins CNP and MBP. However, a significant proportion of immature bipolar GD3+ cells remained after 6 days in secondary culture. It appears that not all the O-2A progenitors in our cultures differentiate immediately and some cells remain in an undifferentiated state and divide to replenish progenitor numbers. We have also identified in our cultures a small apolar GD3- cell, which when isolated differentiated into a GD3+ bipolar O-2A progenitor cell. We have termed this cell type a preprogenitor. The differentiation of this cell type into O-2A progenitors may be the source of the immature GD3+ cells present at the later stages of our secondary cultures. The proliferative profile of the cultures was studied using 5'bromo-2-deoxyuridine (BrdU) incorporation as an index of mitosis. Only the immature, bipolar O-2A progenitors were seen to divide at any time in serum-free culture. Neither the more mature multipolar O-2A cells nor the oligodendroglia were seen to divide. The developmental profile of the O-2A cells in the rat forebrain in vivo showed a largely similar progression to that in culture, with a time lag of at least 6 days between GD3 expression and the onset of myelination. BrdU incorporation studies in vivo also showed that the GD3+ progenitor cell is mitotic whereas the GC(+)-expressing oligodendroglia is not. We have shown that there are several significant alterations in the timing of antigen expression in both O-2A progenitors and oligodendroglia in vitro compared to that seen in vivo.
This article has been cited by other articles:
|
|
 |
|
  E. C. Jacobs, T. M. Pribyl, K. Kampf, C. Campagnoni, C. S. Colwell, S. D. Reyes, M. Martin, V. Handley, T. D. Hiltner, C. Readhead, et al. Region-Specific Myelin Pathology in Mice Lacking the Golli Products of the Myelin Basic Protein Gene J. Neurosci., July 27, 2005; 25(30): 7004 - 7013. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Imitola, E. Y. Snyder, and S. J. Khoury Genetic programs and responses of neural stem/progenitor cells during demyelination: potential insights into repair mechanisms in multiple sclerosis Physiol Genomics, August 15, 2003; 14(3): 171 - 197. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Genoud, C. Lappe-Siefke, S. Goebbels, F. Radtke, M. Aguet, S. S. Scherer, U. Suter, K.-A. Nave, and N. Mantei Notch1 control of oligodendrocyte differentiation in the spinal cord J. Cell Biol., August 19, 2002; 158(4): 709 - 718. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Milner and I. L. Campbell Cytokines Regulate Microglial Adhesion to Laminin and Astrocyte Extracellular Matrix via Protein Kinase C-Dependent Activation of the alpha 6beta 1 Integrin J. Neurosci., March 1, 2002; 22(5): 1562 - 1572. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Lee, V. Lelievre, P. Zhao, M. Torres, W. Rodriguez, J.-Y. Byun, S. Doshi, Y. Ioffe, G. Gupta, A. E. de los Monteros, et al. Pituitary Adenylyl Cyclase-Activating Polypeptide Stimulates DNA Synthesis But Delays Maturation of Oligodendrocyte Progenitors J. Neurosci., June 1, 2001; 21(11): 3849 - 3859. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. L. Follett, P. A. Rosenberg, J. J. Volpe, and F. E. Jensen NBQX Attenuates Excitotoxic Injury in Developing White Matter J. Neurosci., December 15, 2000; 20(24): 9235 - 9241. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Kondo and M. Raff Oligodendrocyte Precursor Cells Reprogrammed to Become Multipotential CNS Stem Cells Science, September 8, 2000; 289(5485): 1754 - 1757. [Abstract] [Full Text]
|
|
|
|
|
|
D. G. Tang, Y. M. Tokumoto, and M. C. Raff Long-Term Culture of Purified Postnatal Oligodendrocyte Precursor Cells: Evidence for an Intrinsic Maturation Program that Plays out over Months J. Cell Biol., March 6, 2000; 148(5): 971 - 984. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Blaschuk, E. Frost, and C ffrench-Constant The regulation of proliferation and differentiation in oligodendrocyte progenitor cells by alphaV integrins Development, January 5, 2000; 127(9): 1961 - 1969. [Abstract] [PDF]
|
|
|
|
|
|
R. Fern and T. Moller Rapid Ischemic Cell Death in Immature Oligodendrocytes: A Fatal Glutamate Release Feedback Loop J. Neurosci., January 1, 2000; 20(1): 34 - 42. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Bansal, S. Winkler, and S. Bheddah Negative Regulation of Oligodendrocyte Differentiation by Galactosphingolipids J. Neurosci., September 15, 1999; 19(18): 7913 - 7924. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. J. Chandross, R. I. Cohen, P. Paras Jr, M. Gravel, P. E. Braun, and L. D. Hudson Identification and Characterization of Early Glial Progenitors Using a Transgenic Selection Strategy J. Neurosci., January 15, 1999; 19(2): 759 - 774. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P Casaccia-Bonnefil, R. Hardy, K. Teng, J. Levine, A Koff, and M. Chao Loss of p27Kip1 function results in increased proliferative capacity of oligodendrocyte progenitors but unaltered timing of differentiation Development, January 9, 1999; 126(18): 4027 - 4037. [Abstract] [PDF]
|
|
|
|
|
|
R. Marmur, J. A. Kessler, G. Zhu, S. Gokhan, and M. F. Mehler Differentiation of Oligodendroglial Progenitors Derived from Cortical Multipotent Cells Requires Extrinsic Signals Including Activation of gp130/LIFbeta Receptors J. Neurosci., December 1, 1998; 18(23): 9800 - 9811. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Ben-Hur, B. Rogister, K. Murray, G. Rougon, and M. Dubois-Dalcq Growth and Fate of PSA-NCAM+ Precursors of the Postnatal Brain J. Neurosci., August 1, 1998; 18(15): 5777 - 5788. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. J. Osterhout, S. Ebner, J. Xu, D. M. Ornitz, G. A. Zazanis, and R. D. McKinnon Transplanted Oligodendrocyte Progenitor Cells Expressing a Dominant-Negative FGF Receptor Transgene Fail to Migrate In Vivo J. Neurosci., December 1, 1997; 17(23): 9122 - 9132. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S.-i. Sakakibara and H. Okano Expression of Neural RNA-Binding Proteins in the Postnatal CNS: Implications of Their Roles in Neuronal and Glial Cell Development J. Neurosci., November 1, 1997; 17(21): 8300 - 8312. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. D. Trapp, A. Nishiyama, D. Cheng, and W. Macklin Differentiation and Death of Premyelinating Oligodendrocytes in Developing Rodent Brain J. Cell Biol., April 21, 1997; 137(2): 459 - 468. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. A. Strait, D. J. Carlson, H. L. Schwartz, and J. H. Oppenheimer Transient Stimulation of Myelin Basic Protein Gene Expression in Differentiating Cultured Oligodendrocytes: A Model for 3,5,3'-Triiodothyronine-Induced Brain Development Endocrinology, February 1, 1997; 138(2): 635 - 641. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Hardy and V. Friedrich Oligodendrocyte progenitors are generated throughout the embryonic mouse brain, but differentiate in restricted foci Development, January 7, 1996; 122(7): 2059 - 2069. [Abstract] [PDF]
|
|
|
|
 |
|
 J. Shioi, M. N. Pangalos, J. A. Ripellino, D. Vassilacopoulou, C. Mytilineou, R. U. Margolis, and N. K. Robakis The Alzheimer Amyloid Precursor Proteoglycan (Appican) Is Present in Brain and Is Produced by Astrocytes but Not by Neurons in Primary Neural Cultures J. Biol. Chem., May 19, 1995; 270(20): 11839 - 11844. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Gard, M. Burrell, S. Pfeiffer, J. Rudge, and W. Williams Astroglial control of oligodendrocyte survival mediated by PDGF and leukemia inhibitory factor-like protein Development, January 7, 1995; 121(7): 2187 - 2197. [Abstract] [PDF]
|
|
|
|
|
|
K Ono, R Bansal, J Payne, U Rutishauser, and R. Miller Early development and dispersal of oligodendrocyte precursors in the embryonic chick spinal cord Development, January 6, 1995; 121(6): 1743 - 1754. [Abstract] [PDF]
|
|
|
|
|
|
B. Barres, M. Lazar, and M. Raff A novel role for thyroid hormone, glucocorticoids and retinoic acid in timing oligodendrocyte development Development, January 5, 1994; 120(5): 1097 - 1108. [Abstract] [PDF]
|
|
|
|
|
|
E Noll and R. Miller Regulation of oligodendrocyte differentiation: a role for retinoic acid in the spinal cord Development, January 3, 1994; 120(3): 649 - 660. [Abstract] [PDF]
|
|
|
|
|
|
M. Raff, B. Barres, J. Burne, H. Coles, Y Ishizaki, and M. Jacobson Programmed cell death and the control of cell survival: lessons from the nervous system Science, October 29, 1993; 262(5134): 695 - 700. [Abstract] [PDF]
|
|
