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Development, Vol 111, Issue 4 933-944, Copyright © 1991 by Company of Biologists
JOURNAL ARTICLES |
A Nishiyama, KJ Dahlin and WB Stallcup
La Jolla Cancer Research Foundation, California 92037.
NG2 is a chondroitin sulfate proteoglycan previously found to be expressed by glial progenitor cells of the O2A lineage. We have examined the expression of NG2 in the developing rat limb by immunohistochemistry and northern blot analysis. Staining of embryonic day 14 (E14) rat limb bud sections with polyclonal and monoclonal anti-NG2 antibodies reveals reactivity in the precartilaginous mesenchymal condensation. The staining intensity increases with the differentiation of chondrocytes until E16. NG2 staining is not detected in the mature hypertrophic chondrocytes of E17 and postnatal day 3 (P3) limbs even after treatment of the sections with hyaluronidase or collagenase. Immuno-precipitations with anti-NG2 antibody using 125I-labeled limb cells in culture showed a 400 to 800 x 10(3) Mr proteoglycan species with a core protein size of 300 x 10(3) Mr, comparable to NG2 from O2A cells and neural cell lines. Northern blot analysis reveals the expression of an 8.9 kb mRNA in E16 limbs and at a lower level in P1 cartilage. The northern blot analyses also show that NG2 is distinct from the large aggregating proteoglycan of the cartilage. Our results indicate that in the developing limb cartilage, as in the differentiating oligodendrocytes, NG2 is present on immature cells in the process of differentiating, but its expression is downregulated as terminal differentiation of chondrocytes takes place.
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  K. Kadoya, J.-i. Fukushi, Y. Matsumoto, Y. Yamaguchi, and W. B. Stallcup NG2 Proteoglycan Expression in Mouse Skin: Altered Postnatal Skin Development in the NG2 Null Mouse J. Histochem. Cytochem., March 1, 2008; 56(3): 295 - 303. [Abstract] [Full Text] [PDF]
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S. R. McGlashan, C. G. Jensen, and C. A. Poole Localization of Extracellular Matrix Receptors on the Chondrocyte Primary Cilium J. Histochem. Cytochem., September 1, 2006; 54(9): 1005 - 1014. [Abstract] [Full Text] [PDF]
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 W. B. Stallcup and K. Dahlin-Huppe Chondroitin sulfate and cytoplasmic domain-dependent membrane targeting of the NG2 proteoglycan promotes retraction fiber formation and cell polarization J. Cell Sci., March 8, 2002; 114(12): 2315 - 2325. [Abstract] [Full Text] [PDF]
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 C. E. Bandtlow and D. R. Zimmermann Proteoglycans in the Developing Brain: New Conceptual Insights for Old Proteins Physiol Rev, October 1, 2000; 80(4): 1267 - 1290. [Abstract] [Full Text] [PDF]
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 Y. Shoshan, A. Nishiyama, A. Chang, S. Mork, G. H. Barnett, J. K. Cowell, B. D. Trapp, and S. M. Staugaitis Expression of oligodendrocyte progenitor cell antigens by gliomas: Implications for the histogenesis of brain tumors PNAS, August 31, 1999; 96(18): 10361 - 10366. [Abstract] [Full Text] [PDF]
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 L. Goretzki, M. A. Burg, K. A. Grako, and W. B. Stallcup High-affinity Binding of Basic Fibroblast Growth Factor and Platelet-derived Growth Factor-AA to the Core Protein of the NG2 Proteoglycan J. Biol. Chem., June 11, 1999; 274(24): 16831 - 16837. [Abstract] [Full Text] [PDF]
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K. Grako, T Ochiya, D Barritt, A Nishiyama, and W. Stallcup PDGF (alpha)-receptor is unresponsive to PDGF-AA in aortic smooth muscle cells from the NG2 knockout mouse J. Cell Sci., January 3, 1999; 112(6): 905 - 915. [Abstract] [PDF]
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A. A. Capehart, M. M. Wienecke, G. T. Kitten, M. Solursh, and E. L. Krug Production of a Monoclonal Antibody by In Vitro Immunization That Recognizes a Native Chondroitin Sulfate Epitope in the Embryonic Chick Limb and Heart J. Histochem. Cytochem., November 1, 1997; 45(11): 1567 - 1582. [Abstract] [Full Text] [PDF]
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E. Tillet, F. Ruggiero, A. Nishiyama, and W. B. Stallcup The Membrane-spanning Proteoglycan NG2 Binds to Collagens V and VI through the Central Nonglobular Domain of Its Core Protein J. Biol. Chem., April 18, 1997; 272(16): 10769 - 10776. [Abstract] [Full Text] [PDF]
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M. A. Burg, E. Tillet, R. Timpl, and W. B. Stallcup Binding of the NG2 Proteoglycan to Type VI Collagen and Other Extracellular Matrix Molecules J. Biol. Chem., October 18, 1996; 271(42): 26110 - 26116. [Abstract] [Full Text] [PDF]
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