|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
Development, Vol 112, Issue 3 881-890, Copyright © 1991 by Company of Biologists
JOURNAL ARTICLES |
O Khaner and F Wilt
Department of Molecular and Cell Biology, University of California, Berkeley 94720.
It has been known from results obtained in the classical experiments on sea urchin embryos that cell isolation and transplantation showed extensive interactions between the early blastomeres and/or their descendants. In the experiments reported here a systematic reexamination of recombination of mesomeres and their progeny (which come from the animal hemisphere) with various vegetal cells derived from blastomeres of the 32- and 64-cell stage was carried out. Cells were marked with lineage tracers to follow which cell gave rise to what structures, and newly available molecular markers have been used to analyze different structures characteristic of regional differentiation. Large micromeres form spicules and induce gut and pigment cells in mesomeres, conforming to previous results. Small micromeres, a cell type not heretofore examined, gave rise to no recognizable structure and had very limited ability to evoke poorly differentiated gut tissue in mesomeres. Macromeres and their descendants, Veg 1 and Veg 2, form primarily what their normal fate dictated, though both did have some capacity to form spicules, presumably by formation from secondary mesenchyme. Macromeres and their descendants were not potent inducers of vegetal structures in animal cells, but they suppress the latent ability of mesomeres to form vegetal structures. The results lead us to propose that the significant interactions during normal development may be principally suppressive effects of mesomeres on one another and of adjacent vegetal cells on mesomeres.
This article has been cited by other articles:
|
|
 |
|
  D. R. Sherwood and D. R. McClay LvNotch signaling plays a dual role in regulating the position of the ectoderm-endoderm boundary in the sea urchin embryo Development, June 15, 2001; 128(12): 2221 - 2232. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Sweet, P. Hodor, and C. Ettensohn The role of micromere signaling in Notch activation and mesoderm specification during sea urchin embryogenesis Development, January 12, 1999; 126(23): 5255 - 5265. [Abstract] [PDF]
|
|
|
|
|
|
C. Logan, J. Miller, M. Ferkowicz, and D. McClay Nuclear beta-catenin is required to specify vegetal cell fates in the sea urchin embryo Development, January 1, 1999; 126(2): 345 - 357. [Abstract] [PDF]
|
|
|
|
|
|
E. Davidson, R. Cameron, and A Ransick Specification of cell fate in the sea urchin embryo: summary and some proposed mechanisms Development, January 9, 1998; 125(17): 3269 - 3290. [Abstract] [PDF]
|
|
|
|
|
|
H Benink, G Wray, and J Hardin Archenteron precursor cells can organize secondary axial structures in the sea urchin embryo Development, January 9, 1997; 124(18): 3461 - 3470. [Abstract] [PDF]
|
|
|
|
|
|
C. Logan and D. McClay The allocation of early blastomeres to the ectoderm and endoderm is variable in the sea urchin embryo Development, January 6, 1997; 124(11): 2213 - 2223. [Abstract] [PDF]
|
|
|
|
|
|
S. Ruffins and C. Ettensohn A fate map of the vegetal plate of the sea urchin (Lytechinus variegatus) mesenchyme blastula Development, January 1, 1996; 122(1): 253 - 263. [Abstract] [PDF]
|
|
|
|
|
|
K. Makabe, C. Kirchhamer, R. Britten, and E. Davidson Cis-regulatory control of the SM50 gene, an early marker of skeletogenic lineage specification in the sea urchin embryo Development, January 7, 1995; 121(7): 1957 - 1970. [Abstract] [PDF]
|
|
|
|
|
|
A. Wikramanayake, B. Brandhorst, and W. Klein Autonomous and non-autonomous differentiation of ectoderm in different sea urchin species Development, January 5, 1995; 121(5): 1497 - 1505. [Abstract] [PDF]
|
|
|
|
 |
|
 A Ransick and E. Davidson A complete second gut induced by transplanted micromeres in the sea urchin embryo Science, February 19, 1993; 259(5098): 1134 - 1138. [Abstract] [PDF]
|
|
|
|
|
|
C. Ettensohn and K. Malinda Size regulation and morphogenesis: a cellular analysis of skeletogenesis in the sea urchin embryo Development, January 9, 1993; 119(1): 155 - 167. [Abstract] [PDF]
|
|
|
|
|
|
E. Davidson Later embryogenesis: regulatory circuitry in morphogenetic fields Development, January 7, 1993; 118(3): 665 - 690. [Abstract] [PDF]
|
|
|
|
|
|
C. Ettensohn and S. Ruffins Mesodermal cell interactions in the sea urchin embryo: properties of skeletogenic secondary mesenchyme cells Development, January 4, 1993; 117(4): 1275 - 1285. [Abstract] [PDF]
|
|
