|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
Development, Vol 114, Issue 1 113-123, Copyright © 1992 by Company of Biologists
JOURNAL ARTICLES |
E Raz and BZ Shilo
Department of Molecular Genetics and Virology, Weizmann Institute of Science, Rehovot, Israel.
The complex embryonic phenotype of mutations in the faint little ball (flb) locus, encoding the Drosophila EGF receptor homolog (DER), was dissected by temperature shifts of a temperature-sensitive allele. We show that the phenotype can be resolved into at least five components, which are temporally and spatially distinct. Most notably, the central nervous system (CNS) phenotype is determined at two separate phases. A severe collapse results from early defects in the DER-expressing ectodermal cells from which neuroblasts and midline glial cells deaminate. We thus suggest that DER activity is crucial for interactions that occur in the ectoderm at an early stage, and determine the fate of neuronal and glial cell lineages. This finding explains how a severe CNS phenotype is generated in flb embryos, in spite of the absence of expression of the protein in neuronal cells. In a second phase, during germ band retraction, the flb function is required specifically in the three pairs of midline glial cells (MG). In the absence of a functional DER protein, these cells die or fail to differentiate correctly, resulting in a fused commissure phenotype.
This article has been cited by other articles:
|
|
 |
|
  H. Yoshida, E. Kwon, F. Hirose, K. Otsuki, M. Yamada, and M. Yamaguchi DREF is required for EGFR signalling during Drosophila wing vein development Genes Cells, October 1, 2004; 9(10): 935 - 944. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. K. Shamloula, M. P. Mbogho, A. C. Pimentel, Z. M. A. Chrzanowska-Lightowlers, V. Hyatt, H. Okano, and T. R. Venkatesh rugose (rg), a Drosophila A kinase Anchor Protein, Is Required for Retinal Pattern Formation and Interacts Genetically With Multiple Signaling Pathways Genetics, June 1, 2002; 161(2): 693 - 710. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Radke, K. Johnson, R. Guo, A. Davidson, and L. Ambrosio Drosophila-Raf Acts to Elaborate Dorsoventral Pattern in the Ectoderm of Developing Embryos Genetics, November 1, 2001; 159(3): 1031 - 1044. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A Amin, Y Li, and R Finkelstein Hedgehog activates the EGF receptor pathway during Drosophila head development Development, January 6, 1999; 126(12): 2623 - 2630. [Abstract] [PDF]
|
|
|
|
|
|
T Hummel, K Schimmelpfeng, and C Klambt Commissure formation in the embryonic CNS of Drosophila Development, January 2, 1999; 126(4): 771 - 779. [Abstract] [PDF]
|
|
|
|
 |
|
 D. Szüts, S. Eresh, and M. Bienz Functional intertwining of Dpp and EGFR signaling during Drosophila endoderm induction Genes & Dev., July 1, 1998; 12(13): 2022 - 2035. [Abstract] [Full Text]
|
|
|
|
|
|
S. T. Crews Control of cell lineage-specific development and transcription by bHLH-PAS proteins Genes & Dev., March 1, 1998; 12(5): 607 - 620. [Full Text]
|
|
|
|
 |
|
 R. Howes, J. D. Wasserman, and M. Freeman In Vivo Analysis of Argos Structure-Function. SEQUENCE REQUIREMENTS FOR INHIBITION OF THE DROSOPHILA EPIDERMAL GROWTH FACTOR RECEPTOR J. Biol. Chem., February 13, 1998; 273(7): 4275 - 4281. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K Dumstrei, C Nassif, G Abboud, A Aryai, A Aryai, and V Hartenstein EGFR signaling is required for the differentiation and maintenance of neural progenitors along the dorsal midline of the Drosophila embryonic head Development, January 9, 1998; 125(17): 3417 - 3426. [Abstract] [PDF]
|
|
|
|
|
|
E Buff, A Carmena, S Gisselbrecht, F Jimenez, and A. Michelson Signalling by the Drosophila epidermal growth factor receptor is required for the specification and diversification of embryonic muscle progenitors Development, January 6, 1998; 125(11): 2075 - 2086. [Abstract] [PDF]
|
|
|
|
|
|
L O'Keefe, S. Dougan, L Gabay, E Raz, B. Shilo, and S DiNardo Spitz and Wingless, emanating from distinct borders, cooperate to establish cell fate across the Engrailed domain in the Drosophila epidermis Development, January 12, 1997; 124(23): 4837 - 4845. [Abstract] [PDF]
|
|
|
|
|
|
T. Haerry, T. Heslip, J. Marsh, and M. O'Connor Defects in glucuronate biosynthesis disrupt Wingless signaling in Drosophila Development, January 8, 1997; 124(16): 3055 - 3064. [Abstract] [PDF]
|
|
|
|
|
|
D Szuts, M Freeman, and M Bienz Antagonism between EGFR and Wingless signalling in the larval cuticle of Drosophila Development, January 8, 1997; 124(16): 3209 - 3219. [Abstract] [PDF]
|
|
|
|
|
|
K Giesen, T Hummel, A Stollewerk, S Harrison, A Travers, and C Klambt Glial development in the Drosophila CNS requires concomitant activation of glial and repression of neuronal differentiation genes Development, January 6, 1997; 124(12): 2307 - 2316. [Abstract] [PDF]
|
|
|
|
|
|
M Freeman Cell determination strategies in the Drosophila eye Development, January 1, 1997; 124(2): 261 - 270. [Abstract] [PDF]
|
|
|
|
|
|
B Schnepp, G Grumbling, T Donaldson, and A Simcox Vein is a novel component in the Drosophila epidermal growth factor receptor pathway with similarity to the neuregulins. Genes & Dev., September 15, 1996; 10(18): 2302 - 2313. [Abstract] [PDF]
|
|
|
|
|
|
L Gabay, H Scholz, M Golembo, A Klaes, B. Shilo, and C Klambt EGF receptor signaling induces pointed P1 transcription and inactivates Yan protein in the Drosophila embryonic ventral ectoderm Development, January 11, 1996; 122(11): 3355 - 3362. [Abstract] [PDF]
|
|
|
|
|
|
L. Frank and C Rushlow A group of genes required for maintenance of the amnioserosa tissue in Drosophila Development, January 5, 1996; 122(5): 1343 - 1352. [Abstract] [PDF]
|
|
|
|
|
|
M Golembo, R Schweitzer, M Freeman, and B. Shilo Argos transcription is induced by the Drosophila EGF receptor pathway to form an inhibitory feedback loop Development, January 1, 1996; 122(1): 223 - 230. [Abstract] [PDF]
|
|
|
|
|
|
R Schweitzer, M Shaharabany, R Seger, and B Z Shilo Secreted Spitz triggers the DER signaling pathway and is a limiting component in embryonic ventral ectoderm determination. Genes & Dev., June 15, 1995; 9(12): 1518 - 1529. [Abstract] [PDF]
|
|
|
|
|
|
L Feng and N Heintz Differentiating neurons activate transcription of the brain lipid-binding protein gene in radial glia through a novel regulatory element Development, January 6, 1995; 121(6): 1719 - 1730. [Abstract] [PDF]
|
|
|
|
|
|
M. A. Sturtevant, J. W. O'Neill, and E. Bier Down-regulation of Drosophila Egf-r mRNA levels following hyperactivated receptor signaling Development, September 1, 1994; 120(9): 2593 - 2600. [Abstract] [PDF]
|
|
|
|
|
|
R Noll, M. Sturtevant, R. Gollapudi, and E Bier New functions of the Drosophila rhomboid gene during embryonic and adult development are revealed by a novel genetic method, enhancer piracy Development, January 8, 1994; 120(8): 2329 - 2338. [Abstract] [PDF]
|
|
|
|
|
|
E Raz and B Z Shilo Establishment of ventral cell fates in the Drosophila embryonic ectoderm requires DER, the EGF receptor homolog. Genes & Dev., October 1, 1993; 7(10): 1937 - 1948. [Abstract] [PDF]
|
|
|
|
|
|
M A Sturtevant, M Roark, and E Bier The Drosophila rhomboid gene mediates the localized formation of wing veins and interacts genetically with components of the EGF-R signaling pathway. Genes & Dev., June 1, 1993; 7(6): 961 - 973. [Abstract] [PDF]
|
|
|
|
|
|
S. Kim and S. Crews Influence of Drosophila ventral epidermal development by the CNS midline cells and spitz class genes Development, January 7, 1993; 118(3): 893 - 901. [Abstract] [PDF]
|
|
|
|
|
|
C Klambt The Drosophila gene pointed encodes two ETS-like proteins which are involved in the development of the midline glial cells Development, January 1, 1993; 117(1): 163 - 176. [Abstract] [PDF]
|
|
|
|
|
|
C Klambt, L Glazer, and B Z Shilo breathless, a Drosophila FGF receptor homolog, is essential for migration of tracheal and specific midline glial cells. Genes & Dev., September 1, 1992; 6(9): 1668 - 1678. [Abstract] [PDF]
|
|
|
|
|
|
B J Rutledge, K Zhang, E Bier, Y N Jan, and N Perrimon The Drosophila spitz gene encodes a putative EGF-like growth factor involved in dorsal-ventral axis formation and neurogenesis. Genes & Dev., August 1, 1992; 6(8): 1503 - 1517. [Abstract] [PDF]
|
|
