|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
Development, Vol 117, Issue 2 641-655, Copyright © 1993 by Company of Biologists
JOURNAL ARTICLES |
EC Martin and PN Adler
Biology Department, University of Virginia, Charlottesville 22903.
The Posterior Sex Combs (Psc) gene of Drosophila has been studied at the molecular level both because it is a Polycomb group (Pc-G) gene and hence required for the maintenance of segmental determination, and because it is the Drosophila homolog of the murine bmi-1 oncogene. Although genetic interactions indicated that Psc functioned as a Pc-G gene, the zygotic mutant phenotype of Psc showed little evidence of segmental transformations. We have examined mutant embryos derived from a mutant maternal germ line and found a stronger mutant phenotype, indicating that the weak zygotic phenotype of Psc is due to maternal rescue. We have found that Psc RNA accumulates in developing oocytes and this maternal RNA is presumably responsible for the maternal rescue. We have studied the expression of the Psc gene at both the RNA and protein levels. On northern blots, we find evidence for two Psc mRNAs and, on western blots, we find evidence for two Psc proteins that are altered either in abundance or size in Psc mutants. The Psc protein accumulates in all regions of the embryo and also in many tissues in a variety of developmental stages. In all cases, it is nuclear, as is its mammalian homolog, the bmi-1 protein. On polytene chromosomes, we find Psc at 45 chromosomal loci where two other Pc-G proteins are present.
This article has been cited by other articles:
|
|
 |
|
  I. Gonzalez, R. Aparicio, and A. Busturia Functional Characterization of the dRYBP Gene in Drosophila Genetics, July 1, 2008; 179(3): 1373 - 1388. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Bello, N. Holbro, and H. Reichert Polycomb group genes are required for neural stem cell survival in postembryonic neurogenesis of Drosophila Development, March 15, 2007; 134(6): 1091 - 1099. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. F. G. King, R. B. Emmons, N. J. Francis, B. Wild, J. Muller, R. E. Kingston, and C.-t. Wu Analysis of a Polycomb Group Protein Defines Regions That Link Repressive Activity on Nucleosomal Templates to In Vivo Function Mol. Cell. Biol., August 1, 2005; 25(15): 6578 - 6591. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Y. Ali and W. Bender Cross-Regulation among the Polycomb Group Genes in Drosophila melanogaster Mol. Cell. Biol., September 1, 2004; 24(17): 7737 - 7747. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. Fischle, Y. Wang, S. A. Jacobs, Y. Kim, C. D. Allis, and S. Khorasanizadeh Molecular basis for the discrimination of repressive methyl-lysine marks in histone H3 by Polycomb and HP1 chromodomains Genes & Dev., August 1, 2003; 17(15): 1870 - 1881. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. L. Brown, C. Fritsch, J. Mueller, and J. A. Kassis The Drosophila pho-like gene encodes a YY1-related DNA binding protein that is redundant with pleiohomeotic in homeotic gene silencing Development, March 2, 2003; 130(2): 285 - 294. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Faucheux, J.-Y. Roignant, S. Netter, J. Charollais, C. Antoniewski, and L. Theodore batman Interacts with Polycomb and trithorax Group Genes and Encodes a BTB/POZ Protein That Is Included in a Complex Containing GAGA Factor Mol. Cell. Biol., February 15, 2003; 23(4): 1181 - 1195. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Mohd-Sarip, F. Venturini, G. E. Chalkley, and C. P. Verrijzer Pleiohomeotic Can Link Polycomb to DNA and Mediate Transcriptional Repression Mol. Cell. Biol., November 1, 2002; 22(21): 7473 - 7483. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y.-L. Chang, Y.-H. Peng, I-C. Pan, D.-S. Sun, B. King, and D.-H. Huang Essential role of Drosophila Hdac1 in homeotic gene silencing PNAS, August 1, 2001; (2001) 171325498. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F Tie, T Furuyama, J Prasad-Sinha, E Jane, and P. Harte The Drosophila Polycomb Group proteins ESC and E(Z) are present in a complex containing the histone-binding protein p55 and the histone deacetylase RPD3 Development, January 1, 2001; 128(2): 275 - 286. [Abstract] [PDF]
|
|
|
|
|
|
J. Ng, C. M. Hart, K. Morgan, and J. A. Simon A Drosophila ESC-E(Z) Protein Complex Is Distinct from Other Polycomb Group Complexes and Contains Covalently Modified ESC Mol. Cell. Biol., May 1, 2000; 20(9): 3069 - 3078. [Abstract] [Full Text]
|
|
|
|
|
|
S. Tillib, S. Petruk, Y. Sedkov, A. Kuzin, M. Fujioka, T. Goto, and A. Mazo Trithorax- and Polycomb-Group Response Elements within an Ultrabithorax Transcription Maintenance Unit Consist of Closely Situated but Separable Sequences Mol. Cell. Biol., July 1, 1999; 19(7): 5189 - 5202. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Bornemann, E. Miller, and J. Simon Expression and Properties of Wild-Type and Mutant Forms of the Drosophila Sex Comb on Midleg (SCM) Repressor Protein Genetics, October 1, 1998; 150(2): 675 - 686. [Abstract] [Full Text]
|
|
|
|
|
|
T. Belenkaya, A. Soldatov, E. Nabirochkina, I. Birjukova, S. Georgieva, and P. Georgiev P-Element Insertion at the polyhomeotic Gene Leads to Formation of a Novel Chimeric Protein That Negatively Regulates yellow Gene Expression in P-Element-Induced Alleles of Drosophila melanogaster Genetics, October 1, 1998; 150(2): 687 - 697. [Abstract] [Full Text]
|
|
|
|
 |
|
 A. J. Saurin, C. Shiels, J. Williamson, D. P.E. Satijn, A. P. Otte, D. Sheer, and P. S. Freemont The Human Polycomb Group Complex Associates with Pericentromeric Heterochromatin to Form a Novel Nuclear Domain J. Cell Biol., August 24, 1998; 142(4): 887 - 898. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
O. Saget, F. Forquignon, P. Santamaria, and N. B. Randsholt Needs and Targets for the multi sex combs Gene Product in Drosophila melanogaster Genetics, August 1, 1998; 149(4): 1823 - 1838. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Kyba and H. W. Brock The Drosophila Polycomb Group Protein Psc Contacts ph and Pc through Specific Conserved Domains Mol. Cell. Biol., May 1, 1998; 18(5): 2712 - 2720. [Abstract] [Full Text]
|
|
|
|
|
|
C. A. Jones, J. Ng, A. J. Peterson, K. Morgan, J. Simon, and R. S. Jones The Drosophila esc and E(z) Proteins Are Direct Partners in Polycomb Group-Mediated Repression Mol. Cell. Biol., May 1, 1998; 18(5): 2825 - 2834. [Abstract] [Full Text]
|
|
|
|
|
|
P. Buchenau, J. Hodgson, H. Strutt, and D. J. Arndt-Jovin The Distribution of Polycomb-Group Proteins During Cell Division and Development in Drosophila Embryos: Impact on Models for Silencing J. Cell Biol., April 20, 1998; 141(2): 469 - 481. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Rosen, D. Dorsett, and J. Jack A Proline-Rich Region in the Zeste Protein Essential for Transvection and white Repression by Zeste1 Genetics, April 1, 1998; 148(4): 1865 - 1874. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K Stankunas, J Berger, C Ruse, D. Sinclair, F Randazzo, and H. Brock The enhancer of polycomb gene of Drosophila encodes a chromatin protein conserved in yeast and mammals Development, January 10, 1998; 125(20): 4055 - 4066. [Abstract] [PDF]
|
|
|
|
|
|
F Tie, T Furuyama, and P. Harte The Drosophila Polycomb Group proteins ESC and E(Z) bind directly to each other and co-localize at multiple chromosomal sites Development, January 9, 1998; 125(17): 3483 - 3496. [Abstract] [PDF]
|
|
|
|
|
|
S Bel, N Core, M Djabali, K Kieboom, N Van der Lugt, M. Alkema, and M Van Lohuizen Genetic interactions and dosage effects of Polycomb group genes in mice Development, January 9, 1998; 125(18): 3543 - 3551. [Abstract] [PDF]
|
|
|
|
|
|
R Holdeman, S Nehrt, and S Strome MES-2, a maternal protein essential for viability of the germline in Caenorhabditis elegans, is homologous to a Drosophila Polycomb group protein Development, January 7, 1998; 125(13): 2457 - 2467. [Abstract] [PDF]
|
|
|
|
|
|
D. Sinclair, T. Milne, J. Hodgson, J Shellard, C. Salinas, M Kyba, F Randazzo, and H. Brock The Additional sex combs gene of Drosophila encodes a chromatin protein that binds to shared and unique Polycomb group sites on polytene chromosomes Development, January 4, 1998; 125(7): 1207 - 1216. [Abstract] [PDF]
|
|
|
|
|
|
Enhancer of Polycomb Is a Suppressor of Position-Effect Variegation in Drosophila melanogaster Genetics, January 1, 1998; 148(1): 211 - 220.
|
|
|
|
|
|
Y Yamamoto, F Girard, B Bello, M Affolter, and W. Gehring The cramped gene of Drosophila is a member of the Polycomb-group, and interacts with mus209, the gene encoding Proliferating Cell Nuclear Antigen Development, January 9, 1997; 124(17): 3385 - 3394. [Abstract] [PDF]
|
|
|
|
|
|
E. Carrington and R. Jones The Drosophila Enhancer of zeste gene encodes a chromosomal protein: examination of wild-type and mutant protein distribution Development, January 12, 1996; 122(12): 4073 - 4083. [Abstract] [PDF]
|
|
|
|
|
|
T Akasaka, M Kanno, R Balling, M. Mieza, M Taniguchi, and H Koseki A role for mel-18, a Polycomb group-related vertebrate gene, during theanteroposterior specification of the axial skeleton Development, January 5, 1996; 122(5): 1513 - 1522. [Abstract] [PDF]
|
|
|
|
|
|
D Bornemann, E Miller, and J Simon The Drosophila Polycomb group gene Sex comb on midleg (Scm) encodes a zinc finger protein with similarity to polyhomeotic protein Development, January 5, 1996; 122(5): 1621 - 1630. [Abstract] [PDF]
|
|
|
|
|
|
J Muller, S Gaunt, and P. Lawrence Function of the Polycomb protein is conserved in mice and flies Development, January 9, 1995; 121(9): 2847 - 2852. [Abstract] [PDF]
|
|
|
|
|
|
A Chiang, M. O'Connor, R Paro, J Simon, and W Bender Discrete Polycomb-binding sites in each parasegmental domain of the bithorax complex Development, January 6, 1995; 121(6): 1681 - 1689. [Abstract] [PDF]
|
|
|
|
|
|
B Kuzin, S Tillib, Y Sedkov, L Mizrokhi, and A Mazo The Drosophila trithorax gene encodes a chromosomal protein and directly regulates the region-specific homeotic gene fork head. Genes & Dev., October 15, 1994; 8(20): 2478 - 2490. [Abstract] [PDF]
|
|
|
|
|
|
A. Lonie, R. D'Andrea, R. Paro, and R. Saint Molecular characterisation of the Polycomblike gene of Drosophila melanogaster, a trans-acting negative regulator of homeotic gene expression Development, September 1, 1994; 120(9): 2629 - 2636. [Abstract] [PDF]
|
|
|
|
|
|
N M van der Lugt, J Domen, K Linders, M van Roon, E Robanus-Maandag, H te Riele, M van der Valk, J Deschamps, M Sofroniew, and M van Lohuizen Posterior transformation, neurological abnormalities, and severe hematopoietic defects in mice with a targeted deletion of the bmi-1 proto-oncogene. Genes & Dev., April 1, 1994; 8(7): 757 - 769. [Abstract] [PDF]
|
|
|
|
 |
|
 P. Singh Molecular mechanisms of cellular determination: their relation to chromatin structure and parental imprinting J. Cell Sci., January 10, 1994; 107(10): 2653 - 2668. [PDF]
|
|
|
|
|
|
Y Sedkov, S Tillib, L Mizrokhi, and A Mazo The bithorax complex is regulated by trithorax earlier during Drosophila embryogenesis than is the Antennapedia complex, correlating with a bithorax-like expression pattern of distinct early trithorax transcripts Development, January 7, 1994; 120(7): 1907 - 1917. [Abstract] [PDF]
|
|
|
|
|
|
Y.-L. Chang, Y.-H. Peng, I-C. Pan, D.-S. Sun, B. King, and D.-H. Huang Essential role of Drosophila Hdac1 in homeotic gene silencing PNAS, August 14, 2001; 98(17): 9730 - 9735. [Abstract] [Full Text] [PDF]
|
|
