|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
Development, Vol 118, Issue 3 845-858, Copyright © 1993 by Company of Biologists
JOURNAL ARTICLES |
GH Yee and RO Hynes
Howard Hughes Medical Institute, Massachusetts Institute of Technology, Cambridge 02139.
The integrins are a family of cell surface receptors for extracellular matrix proteins and counter-receptors on other cells. We have used the polymerase chain reaction to identify a novel integrin receptor beta subunit in Drosophila melanogaster. The deduced amino acid sequence of this subunit, which we have termed beta v (beta-neu), indicates that it has several unusual properties. The beta v subunit is roughly 33% identical with each of the previously sequenced vertebrate and Drosophila beta subunits and is lacking four of the 56 cysteine residues characteristic of most members of this protein family. The expression of the beta v gene is strikingly restricted. It is temporally regulated, with maximal expression occurring at 12-15 hours of embryonic development. In situ hybridization analyses and antibody localization on whole-mount embryos reveal that beta v expression is tissue-specific and confined to the developing midgut endoderm and its precursors during embryogenesis. Tissue specificity of expression is maintained through later stages of development as beta v transcripts are found exclusively in the larval midgut. Within this structure, beta v transcripts are especially concentrated in the cells of the midgut imaginal islands which give rise to the adult midgut.
This article has been cited by other articles:
|
|
 |
|
  Z. Huang, U. Yazdani, K. L. Thompson-Peer, A. L. Kolodkin, and J. R. Terman Crk-associated substrate (Cas) signaling protein functions with integrins to specify axon guidance during development Development, June 15, 2007; 134(12): 2337 - 2347. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Ohkawa, J. O. Washburn, R. Sitapara, E. Sid, and L. E. Volkman Specific Binding of Autographa californica M Nucleopolyhedrovirus Occlusion-Derived Virus to Midgut Cells of Heliothis virescens Larvae Is Mediated by Products of pif Genes Ac119 and Ac022 but Not by Ac115 J. Virol., December 15, 2005; 79(24): 15258 - 15264. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Devenport and N. H. Brown Morphogenesis in the absence of integrins: mutation of both Drosophila {beta} subunits prevents midgut migration Development, November 1, 2004; 131(21): 5405 - 5415. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Hayashi, M. Hayashi, and S. Kobayashi Nanos suppresses somatic cell fate in Drosophila germ line PNAS, July 13, 2004; 101(28): 10338 - 10342. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. J. Jaffe, A. C. Leopold, and R. C. Staples Thigmo responses in plants and fungi Am. J. Botany, March 1, 2002; 89(3): 375 - 382. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Miyazawa, K. Azumi, and M. Nonaka Cloning and Characterization of Integrin {{alpha}} Subunits from the Solitary Ascidian, Halocynthia roretzi J. Immunol., February 1, 2001; 166(3): 1710 - 1715. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Martin-Bermudo and N. Brown The localized assembly of extracellular matrix integrin ligands requires cell-cell contact J. Cell Sci., January 11, 2000; 113(21): 3715 - 3723. [Abstract] [PDF]
|
|
|
|
 |
|
 R. H. Palmer, L. I. Fessler, P. T. Edeen, S. J. Madigan, M. McKeown, and T. Hunter DFak56 Is a Novel Drosophila melanogaster Focal Adhesion Kinase J. Biol. Chem., December 10, 1999; 274(50): 35621 - 35629. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. D. Martin-Bermudo and N. H. Brown Uncoupling integrin adhesion and signaling: the beta PS cytoplasmic domain is sufficient to regulate gene expression in the Drosophila embryo Genes & Dev., March 15, 1999; 13(6): 729 - 739. [Abstract] [Full Text]
|
|
|
|
|
|
K. Beumer, J Rohrbough, A Prokop, and K Broadie A role for PS integrins in morphological growth and synaptic function at the postembryonic neuromuscular junction of Drosophila Development, January 12, 1999; 126(24): 5833 - 5846. [Abstract] [PDF]
|
|
|
|
 |
|
 B. Hoang and A. Chiba Genetic Analysis on the Role of Integrin during Axon Guidance in Drosophila J. Neurosci., October 1, 1998; 18(19): 7847 - 7855. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. L. Brower, S. M. Brower, D. C. Hayward, and E. E. Ball Molecular evolution of integrins: Genes encoding integrin beta subunits from a coral and a sponge PNAS, August 19, 1997; 94(17): 9182 - 9187. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Brown Null mutations in the alpha PS2 and beta PS integrin subunit genes have distinct phenotypes Development, January 5, 1994; 120(5): 1221 - 1231. [Abstract] [PDF]
|
|
|
|
|
|
U Tepass and V Hartenstein Epithelium formation in the Drosophila midgut depends on the interaction of endoderm and mesoderm Development, January 3, 1994; 120(3): 579 - 590. [Abstract] [PDF]
|
|
|
|
|
|
Y Grinblat, S Zusman, G Yee, R. Hynes, and F. Kafatos Functions of the cytoplasmic domain of the beta PS integrin subunit during Drosophila development Development, January 1, 1994; 120(1): 91 - 102. [Abstract] [PDF]
|
|
|
|
|
|
R. Reuter, B. Grunewald, and M. Leptin A role for the mesoderm in endodermal migration and morphogenesis in Drosophila Development, December 1, 1993; 119(4): 1135 - 1145. [Abstract] [PDF]
|
|
