|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
Development, Vol 120, Issue 10 2891-2900, Copyright © 1994 by Company of Biologists
JOURNAL ARTICLES |
DL Richardson, WF Loomis and AR Kimmel
Laboratory of Cellular and Developmental Biology, NIDDK, NIH, Bethesda, MD 20892, USA.
spiA, a marker for sporulation, is expressed during the culmination stage of Dictyostelium development, when the mass of prespore cells has moved partly up the newly formed stalk. Strains containing a full-length spiA promoter/lacZ fusion were stained for beta-galactosidase activity at intervals during development. The results indicate that expression of spiA initiates in prespore cells at the prestalk/prespore boundary (near the apex) and extends downward into the prespore mass as culmination continues. A spatial gradient of staining expands from the top of the prespore mass and intensifies until the front of activation reaches the bottom, whereupon the entire region stains darkly. The spiA promoter can be deleted to within 301 bp of the transcriptional start site with no effect on the relative strength, timing or spatial localization of expression. Further 5' deletions from -301 to -175 reduce promoter strength incrementally, although timing and spatial expression are not affected. Deletions to -159 and beyond result in inactive promoters. Treatment of early developmental structures with 8-Br-cAMP in situ activates the intracellular cAMP-dependent protein kinase (PKA) and precociously induces spiA expression and sporulation. The absence of an apparent gradient of staining in these structures suggest that PKA is equivalently activatable throughout the prespore region and that all prespore cells are competent to express spiA. Thus, we postulate that the pattern of expression of spiA reveals the progression of an inductive signal for sporulation and suggest that this signal may originate from the prestalk cells at the apex.
This article has been cited by other articles:
|
|
 |
|
  C. M. West, H. van der Wel, and Z. A. Wang Prolyl 4-hydroxylase-1 mediates O2 signaling during development of Dictyostelium Development, September 15, 2007; 134(18): 3349 - 3358. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Metcalf, H. van der Wel, R. Escalante, L. Sastre, and C. M. West Role of SP65 in Assembly of the Dictyostelium discoideum Spore Coat Eukaryot. Cell, July 1, 2007; 6(7): 1137 - 1149. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. K. Singleton, J. H. Kirsten, and C. J. Dinsmore Function of Ammonium Transporter A in the Initiation of Culmination of Development in Dictyostelium discoideum. Eukaryot. Cell, July 1, 2006; 5(7): 991 - 996. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Anjard and W. F. Loomis Peptide signaling during terminal differentiation of Dictyostelium PNAS, May 24, 2005; 102(21): 7607 - 7611. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Maruo, H. Sakamoto, N. Iranfar, D. Fuller, T. Morio, H. Urushihara, Y. Tanaka, M. Maeda, and W. F. Loomis Control of Cell Type Proportioning in Dictyostelium discoideum by Differentiation-Inducing Factor as Determined by In Situ Hybridization Eukaryot. Cell, October 1, 2004; 3(5): 1241 - 1248. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. R. Good, M. Cabral, S. Sharma, J. Yang, N. Van Driessche, C. A. Shaw, G. Shaulsky, and A. Kuspa TagA, a putative serine protease/ABC transporter of Dictyostelium that is required for cell fate determination at the onset of development Development, July 1, 2003; 130(13): 2953 - 2965. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. M. West, P. Zhang, A. C. McGlynn, and L. Kaplan Outside-In Signaling of Cellulose Synthesis by a Spore Coat Protein in Dictyostelium Eukaryot. Cell, April 1, 2002; 1(2): 281 - 292. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Wang, F. Soderbom, C. Anjard, G. Shaulsky, and W. F. Loomis SDF-2 Induction of Terminal Differentiation in Dictyostelium discoideum Is Mediated by the Membrane-Spanning Sensor Kinase DhkA Mol. Cell. Biol., July 1, 1999; 19(7): 4750 - 4756. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F Soderbom, C Anjard, N Iranfar, D Fuller, and W. Loomis An adenylyl cyclase that functions during late development of Dictyostelium Development, January 12, 1999; 126(23): 5463 - 5471. [Abstract] [PDF]
|
|
|
|
|
|
K Guo, C Anjard, A Harwood, H. Kim, P. Newell, and J. Gross A myb-related protein required for culmination in Dictyostelium Development, January 6, 1999; 126(12): 2813 - 2822. [Abstract] [PDF]
|
|
|
|
 |
|
 S. Pukatzki, N. Tordilla, J. Franke, and R. H. Kessin A Novel Component Involved in Ubiquitination Is Required for Development of Dictyostelium discoideum J. Biol. Chem., September 11, 1998; 273(37): 24131 - 24138. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. F. Loomis Role of PKA in the Timing of Developmental Events in Dictyostelium Cells Microbiol. Mol. Biol. Rev., September 1, 1998; 62(3): 684 - 694. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Aubry and R. A. Firtel Spalten, a protein containing Galpha -protein-like and PP2C domains, is essential for cell-type differentiation in Dictyostelium Genes & Dev., May 15, 1998; 12(10): 1525 - 1538. [Abstract] [Full Text]
|
|
|
|
|
|
P Balint-Kurti, G. Ginsburg, J Liu, and A. Kimmel Non-autonomous regulation of a graded, PKA-mediated transcriptional activation signal for cell patterning Development, January 10, 1998; 125(20): 3947 - 3954. [Abstract] [PDF]
|
|
|
|
|
|
C Anjard, W. Chang, J Gross, and W Nellen Production and activity of spore differentiation factors (SDFs) in Dictyostelium Development, January 10, 1998; 125(20): 4067 - 4075. [Abstract] [PDF]
|
|
|
|
|
|
X Mu, B Lee, J. Louis, and A. Kimmel Sequence-specific protein interaction with a transcriptional enhancer involved in the autoregulated expression of cAMP receptor 1 in Dictyostelium Development, January 9, 1998; 125(18): 3689 - 3698. [Abstract] [PDF]
|
|
|
|
 |
|
 A. Clark, A. Nomura, S. Mohanty, and R. A. Firtel A Ubiquitin-Conjugating Enzyme Is Essential for Developmental Transitions in Dictyostelium Mol. Biol. Cell, October 1, 1997; 8(10): 1989 - 2002. [Abstract] [Full Text]
|
|
|
|
|
|
G. T. Ginsburg and A. R. Kimmel Autonomous and nonautonomous regulation of axis formation by antagonistic signaling via 7-span cAMP receptors and GSK3 in Dictyostelium Genes & Dev., August 15, 1997; 11(16): 2112 - 2123. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Chaumont, W. F. Loomis, and M. J. Chrispeels Expression of an Arabidopsis plasma membrane aquaporin in Dictyostelium results in hypoosmotic sensitivity and developmental abnormalities PNAS, June 10, 1997; 94(12): 6202 - 6209. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S Lee, R Escalante, and R. Firtel A Ras GAP is essential for cytokinesis and spatial patterning in Dictyostelium Development, January 3, 1997; 124(5): 983 - 996. [Abstract] [PDF]
|
|
|
|
|
|
P Balint-Kurti, G Ginsburg, O Rivero-Lezcano, and A. Kimmel rZIP, a RING-leucine zipper protein that regulates cell fate determination during Dictyostelium development Development, January 3, 1997; 124(6): 1203 - 1213. [Abstract] [PDF]
|
|
|
|
|
|
G. Shaulsky, R. Escalante, and W. F. Loomis Developmental signal transduction pathways uncovered by genetic suppressors PNAS, December 24, 1996; 93(26): 15260 - 15265. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Nuckolls, N Osherov, W. Loomis, and J. Spudich The Dictyostelium dual-specificity kinase splA is essential for spore differentiation Development, January 10, 1996; 122(10): 3295 - 3305. [Abstract] [PDF]
|
|
|
|
|
|
R A Firtel Integration of signaling information in controlling cell-fate decisions in Dictyostelium. Genes & Dev., June 15, 1995; 9(12): 1427 - 1444. [PDF]
|
|
|
|
|
|
G Shaulsky, A Kuspa, and W F Loomis A multidrug resistance transporter/serine protease gene is required for prestalk specialization in Dictyostelium. Genes & Dev., May 1, 1995; 9(9): 1111 - 1122. [Abstract] [PDF]
|
|
|
|
|
|
H. L. Ennis, D. N. Dao, S. U. Pukatzki, and R. H. Kessin Dictyostelium amoebae lacking an F-box protein form spores rather than stalk in chimeras with wild type PNAS, March 28, 2000; 97(7): 3292 - 3297. [Abstract] [Full Text] [PDF]
|
|
