|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
Development, Vol 120, Issue 7 1861-1872, Copyright © 1994 by Company of Biologists
JOURNAL ARTICLES |
JA White, MB Boffa, B Jones and M Petkovich
Department of Pathology, Queen's University, Kingston, Ontario, Canada.
Retinoic acid (RA) is an important signalling molecule in vertebrate pattern formation both in developing and regenerating tissues. The effects of RA are due largely to regulation of gene transcription, mediated by retinoic acid receptors (RAR-alpha, RAR-beta, RAR-gamma) and retinoid X receptors (RXR-alpha, RXR-beta, RXR-gamma). We have been using zebrafish as a model of regeneration to study the role of retinoic acid and its receptors in vertebrate pattern formation. In this report, we describe the molecular cloning and characterization of one of the zebrafish RARs that is the predominant receptor in the regenerating caudal fin and corresponds most closely to the RAR-gamma subtype isolated from mouse and human and to RAR-delta from newt. Zebrafish RAR-gamma (zfRAR-gamma) exhibits both structural and functional conservation with its mammalian counterparts. Studies utilizing both normal and regenerating caudal fins of the zebrafish have indicated that it is the RAR-gamma subtype, compared to RAR-alpha or RAR-beta, which is expressed at the highest levels in the tail fin. To localize the expression pattern of RAR-gamma during fin regeneration, we have carried out whole-mount in situ hybridization. ZfRAR-gamma transcripts, during fin regeneration, are localized in the blastemal tissue formed at the distal ends of the bony rays following amputation. Treatment of fish with RA during fin regeneration induces a number of striking morphological effects on the regenerate. When amputations are performed distal to the branch points or dichotomies, where a single ray bifurcates to extend two individual 'daughter' rays, RA treatment causes a dichotomy reduction where the two 'daughter' rays fuse to once again form a single ray. The single ray subsequently bifurcates in a comparatively normal manner. Our data suggest that exogenous RA can respecify pattern in the regenerating caudal fin and identifies the blastemae as possible RA target tissues.
This article has been cited by other articles:
|
|
 |
|
  C. L. Stoick-Cooper, R. T. Moon, and G. Weidinger Advances in signaling in vertebrate regeneration as a prelude to regenerative medicine Genes & Dev., June 1, 2007; 21(11): 1292 - 1315. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. K. Mathew, E. A. Andreasen, and R. L. Tanguay Aryl Hydrocarbon Receptor Activation Inhibits Regenerative Growth Mol. Pharmacol., January 1, 2006; 69(1): 257 - 265. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Lee, S. Grill, A. Sanchez, M. Murphy-Ryan, and K. D. Poss Fgf signaling instructs position-dependent growth rate during zebrafish fin regeneration Development, December 1, 2005; 132(23): 5173 - 5183. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Holzschuh, A. Barrallo-Gimeno, A.-K. Ettl, K. Durr, E. W. Knapik, and W. Driever Noradrenergic neurons in the zebrafish hindbrain are induced by retinoic acid and require tfap2a for expression of the neurotransmitter phenotype Development, December 1, 2003; 130(23): 5741 - 5754. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. M. Zodrow and R. L. Tanguay 2,3,7,8-Tetrachlorodibenzo-p-dioxin Inhibits Zebrafish Caudal Fin Regeneration Toxicol. Sci., November 1, 2003; 76(1): 151 - 161. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. B. Boffa, J. D. Hamill, N. Bastajian, R. Dillon, M. E. Nesheim, and M. L. Koschinsky A Role for CCAAT/Enhancer-binding Protein in Hepatic Expression of Thrombin-activable Fibrinolysis Inhibitor J. Biol. Chem., July 5, 2002; 277(28): 25329 - 25336. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 O. Loudig, C. Babichuk, J. White, S. Abu-Abed, C. Mueller, and M. Petkovich Cytochrome P450RAI(CYP26) Promoter: A Distinct Composite Retinoic Acid Response Element Underlies the Complex Regulation of Retinoic Acid Metabolism Mol. Endocrinol., September 1, 2000; 14(9): 1483 - 1497. [Abstract] [Full Text]
|
|
|
|
|
|
S. S. Abu-Abed, B. R. Beckett, H. Chiba, J. V. Chithalen, G. Jones, D. Metzger, P. Chambon, and M. Petkovich Mouse P450RAI (CYP26) Expression and Retinoic Acid-inducible Retinoic Acid Metabolism in F9 Cells Are Regulated by Retinoic Acid Receptor gamma and Retinoid X Receptor alpha J. Biol. Chem., January 23, 1998; 273(4): 2409 - 2415. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L Laforest, C. Brown, G Poleo, J Geraudie, M Tada, M Ekker, and M. Akimenko Involvement of the sonic hedgehog, patched 1 and bmp2 genes in patterning of the zebrafish dermal fin rays Development, January 11, 1998; 125(21): 4175 - 4184. [Abstract] [PDF]
|
|
|
|
 |
|
 D. E. Cash, C. B. Bock, K. Schughart, E. Linney, and T. M. Underhill Retinoic Acid Receptor alpha Function in Vertebrate Limb Skeletogenesis: a Modulator of Chondrogenesis J. Cell Biol., January 27, 1997; 136(2): 445 - 457. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. A. White, Y.-D. Guo, K. Baetz, B. Beckett-Jones, J. Bonasoro, K. E. Hsu, F. J. Dilworth, G. Jones, and M. Petkovich Identification of the Retinoic Acid-inducible All-trans-retinoic Acid 4-Hydroxylase J. Biol. Chem., November 22, 1996; 271(47): 29922 - 29927. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Akimenko, S. Johnson, M Westerfield, and M Ekker Differential induction of four msx homeobox genes during fin development and regeneration in zebrafish Development, January 2, 1995; 121(2): 347 - 357. [Abstract] [PDF]
|
|
