|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
Development, Vol 121, Issue 2 287-297, Copyright © 1995 by Company of Biologists
JOURNAL ARTICLES |
M Kloc and LD Etkin
Department of Molecular Genetics, University of Texas M.D. Anderson Cancer Center, Houston 77030, USA.
We found that there are two major pathways by which RNAs are localized at the vegetal cortex during oogenesis of Xenopus laevis. One of these, through which Xlsirts, Xcat2 and Xwnt11 are localized, involves transport during stages 1 and 2 of oogenesis via a region of the mitochondrial cloud that we call the message transport organizer or METRO. This pathway involved three steps, transport of RNA from the GV to the mitochondrial cloud, sorting of the RNAs to specific regions of the METRO, and translocation to and anchoring at the vegetal cortex. These three RNAs exhibit a distinct pattern of spatial localization within the METRO when they approach the vegetal cortex. The other pathway is used by Vg1. We detected Vg1 throughout the oocyte cytoplasm during stages 1 and 2. During stage 3 it was translocated to the vegetal cortex and associated with the cortex overlapping the region at which the Xlsirt, Xcat2, and Xwnt11 RNAs are anchored. Our results also showed that anchoring of these RNAs was dependent in part on actin microfilaments, but was independent of microtubules. These results demonstrate a novel mechanism of translocation and RNA sorting used by RNAs several of which may be involved in the establishment of the embryonic body axis.
This article has been cited by other articles:
|
|
 |
|
  M. E. Pepling, J. E. Wilhelm, A. L. O'Hara, G. W. Gephardt, and A. C. Spradling Mouse oocytes within germ cell cysts and primordial follicles contain a Balbiani body PNAS, January 2, 2007; 104(1): 187 - 192. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. V. Danilchik, E. E. Brown, and K. Riegert Intrinsic chiral properties of the Xenopus egg cortex: an early indicator of left-right asymmetry? Development, November 15, 2006; 133(22): 4517 - 4526. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Kloc, K. Wilk, D. Vargas, Y. Shirato, S. Bilinski, and L. D. Etkin Potential structural role of non-coding and coding RNAs in the organization of the cytoskeleton at the vegetal cortex of Xenopus oocytes Development, August 1, 2005; 132(15): 3445 - 3457. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Kloc and L. D. Etkin RNA localization mechanisms in oocytes J. Cell Sci., January 15, 2005; 118(2): 269 - 282. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Chang, J. Torres, R. A. Lewis, K. L. Mowry, E. Houliston, and M. L. King Localization of RNAs to the Mitochondrial Cloud in Xenopus Oocytes through Entrapment and Association with Endoplasmic Reticulum Mol. Biol. Cell, October 1, 2004; 15(10): 4669 - 4681. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. J. Yoon and K. L. Mowry Xenopus Staufen is a component of a ribonucleoprotein complex containing Vg1 RNA and kinesin Development, July 1, 2004; 131(13): 3035 - 3045. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Hamada, K. Ishiyama, S.-B. Choi, C. Wang, S. Singh, N. Kawai, V. R. Franceschi, and T. W. Okita The Transport of Prolamine RNAs to Prolamine Protein Bodies in Living Rice Endosperm Cells PLANT CELL, October 1, 2003; 15(10): 2253 - 2264. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. T. Cox and A. C. Spradling A Balbiani body and the fusome mediate mitochondrial inheritance during Drosophila oogenesis Development, April 15, 2003; 130(8): 1579 - 1590. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. T. Kroll, W.-m. Zhao, C. Jiang, and P. W. Huber A homolog of FBP2/KSRP binds to localized mRNAs in Xenopus oocytes Development, March 14, 2003; 129(24): 5609 - 5619. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Nakamura, R. Amikura, K. Hanyu, and S. Kobayashi Me31B silences translation of oocyte-localizing RNAs through the formation of cytoplasmic RNP complex during Drosophila oogenesis Development, September 1, 2001; 128(17): 3233 - 3242. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Alarcon and R. Elinson RNA anchoring in the vegetal cortex of the Xenopus oocyte J. Cell Sci., January 5, 2001; 114(9): 1731 - 1741. [Abstract] [PDF]
|
|
|
|
 |
|
 P. Mas and R. N. Beachy Replication of Tobacco Mosaic Virus on Endoplasmic Reticulum and Role of the Cytoskeleton and Virus Movement Protein in Intracellular Distribution of Viral RNA J. Cell Biol., November 29, 1999; 147(5): 945 - 958. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R.G. Edwards and H. K. Beard Hypothesis: sex determination and germline formation are committed at the pronucleate stage in mammalian embryos Mol. Hum. Reprod., July 1, 1999; 5(7): 595 - 606. [Full Text] [PDF]
|
|
|
|
 |
|
 K. M. Bhat The Posterior Determinant Gene nanos Is Required for the Maintenance of the Adult Germline Stem Cells During Drosophila Oogenesis Genetics, April 1, 1999; 151(4): 1479 - 1492. [Abstract] [Full Text]
|
|
|
|
|
|
A. Chan, M Kloc, and L. Etkin fatvg encodes a new localized RNA that uses a 25-nucleotide element (FVLE1) to localize to the vegetal cortex of Xenopus oocytes Development, January 11, 1999; 126(22): 4943 - 4953. [Abstract] [PDF]
|
|
|
|
|
|
T Nakadai, T Kishimoto, Y Miyazawa, N Okada, Y Makino, T Obinata, and T Tamura HP33: hepatocellular carcinoma-enriched 33-kDa protein with similarity to mitochondrial N-acyltransferase but localized in a microtubule-dependent manner at the centrosome J. Cell Sci., January 5, 1999; 112(9): 1353 - 1364. [Abstract] [PDF]
|
|
|
|
 |
|
 L. Havin, A. Git, Z. Elisha, F. Oberman, K. Yaniv, S. P. Schwartz, N. Standart, and J. K. Yisraeli RNA-binding protein conserved in both microtubule- and microfilament-based RNA localization Genes & Dev., June 1, 1998; 12(11): 1593 - 1598. [Abstract] [Full Text]
|
|
|
|
|
|
R Martin, B Vaida, R Bleher, M Crispino, and A Giuditta Protein synthesizing units in presynaptic and postsynaptic domains of squid neurons J. Cell Sci., January 11, 1998; 111(21): 3157 - 3166. [Abstract] [PDF]
|
|
|
|
|
|
M. Wilsch-Brauninger, H. Schwarz, and C. Nusslein-Volhard A Sponge-like Structure Involved in the Association and Transport of Maternal Products during Drosophila Oogenesis J. Cell Biol., November 3, 1997; 139(3): 817 - 829. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D Gautreau, C. Cote, and K. Mowry Two copies of a subelement from the Vg1 RNA localization sequence are sufficient to direct vegetal localization in Xenopus oocytes Development, January 12, 1997; 124(24): 5013 - 5020. [Abstract] [PDF]
|
|
|
|
|
|
M. Horb and G. Thomsen A vegetally localized T-box transcription factor in Xenopus eggs specifies mesoderm and endoderm and is essential for embryonic mesoderm formation Development, January 5, 1997; 124(9): 1689 - 1698. [Abstract] [PDF]
|
|
|
|
|
|
H Kageura Activation of dorsal development by contact between the cortical dorsal determinant and the equatorial core cytoplasm in eggs of Xenopus laevis Development, January 4, 1997; 124(8): 1543 - 1551. [Abstract] [PDF]
|
|
|
|
|
|
Y Zhou and M. King Localization of Xcat-2 RNA, a putative germ plasm component, to the mitochondrial cloud in Xenopus stage I oocytes Development, January 9, 1996; 122(9): 2947 - 2953. [Abstract] [PDF]
|
|
|
|
 |
|
 S. Runge, F. C. Nielsen, J. Nielsen, J. Lykke-Andersen, U. M. Wewer, and J. Christiansen H19 RNA Binds Four Molecules of Insulin-like Growth Factor II mRNA-binding Protein J. Biol. Chem., September 15, 2000; 275(38): 29562 - 29569. [Abstract] [Full Text] [PDF]
|
|
