|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
Development, Vol 121, Issue 4 935-945, Copyright © 1995 by Company of Biologists
JOURNAL ARTICLES |
E Birgbauer, J Sechrist, M Bronner-Fraser and S Fraser
Division of Biology, California Institute of Technology, Pasadena 91125, USA.
Neural crest cell migration in the hindbrain is segmental, with prominent streams of migrating cells adjacent to rhombomeres (r) r2, r4 and r6, but not r3 or r5. This migratory pattern cannot be explained by the failure of r3 and r5 to produce neural crest, since focal injections of the lipophilic dye, DiI, into the neural folds clearly demonstrate that all rhombomeres produce neural crest cells. Here, we examine the dynamics of hindbrain neural crest cell emigration and movement by iontophoretically injecting DiI into small numbers of cells. The intensely labeled cells and their progeny were repeatedly imaged using low-light-level epifluorescence microscopy, permitting their movement to be followed in living embryos over time. These intravital images definitively show that neural crest cells move both rostrally and caudally from r3 and r5 to emerge as a part of the streams adjacent to r2, r4, and/or r6. Within the first few hours, cells labeled in r3 move within and/or along the dorsal neural tube surface, either rostrally toward the r2/3 border or caudally toward the r3/4 border. The labeled cells exit the surface of the neural tube near these borders and migrate toward the first or second branchial arches several hours after initial labeling. Focal DiI injections into r5 resulted in neural crest cell contributions to both the second and third branchial arches, again via rostrocaudal movements of the cells before migration into the periphery. These results demonstrate conclusively that all rhombomeres give rise to neural crest cells, and that rostrocaudal rearrangement of the cells contributes to the segmental migration of neural crest cells adjacent to r2, r4, and r6. Furthermore, it appears that there are consistent exit points of neural crest cell emigration; for example, cells arising from r3 emigrate almost exclusively from the rostral or caudal borders of that rhombomere.
This article has been cited by other articles:
|
|
 |
|
  D. Chomette, M. Frain, S. Cereghini, P. Charnay, and J. Ghislain Krox20 hindbrain cis-regulatory landscape: interplay between multiple long-range initiation and autoregulatory elements Development, April 1, 2006; 133(7): 1253 - 1262. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. M. Kulesa, J. C. Kasemeier-Kulesa, J. M. Teddy, N. V. Margaryan, E. A. Seftor, R. E. B. Seftor, and M. J. C. Hendrix Reprogramming metastatic melanoma cells to assume a neural crest cell-like phenotype in an embryonic microenvironment PNAS, March 7, 2006; 103(10): 3752 - 3757. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. M. Teddy and P. M. Kulesa In vivo evidence for short- and long-range cell communication in cranial neural crest cells Development, December 15, 2004; 131(24): 6141 - 6151. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. M. Le Douarin, S. Creuzet, G. Couly, and E. Dupin Neural crest cell plasticity and its limits Development, October 1, 2004; 131(19): 4637 - 4650. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Perez-Alcala, M. A. Nieto, and J. A. Barbas LSox5 regulates RhoB expression in the neural tube and promotes generation of the neural crest Development, September 15, 2004; 131(18): 4455 - 4465. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. W. McCauley and M. Bronner-Fraser Neural crest contributions to the lamprey head Development, June 1, 2003; 130(11): 2317 - 2327. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. U. Frank, L. K. Fotheringham, J. A. Brewer, L. J. Muglia, M. Tristani-Firouzi, M. R. Capecchi, and A. M. Moon An Fgf8 mouse mutant phenocopies human 22q11 deletion syndrome Development, January 10, 2002; 129(19): 4591 - 4603. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. G. del Barrio and M. A. Nieto Overexpression of Snail family members highlights their ability to promote chick neural crest formation Development, January 4, 2002; 129(7): 1583 - 1593. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Etchevers, C Vincent, N. Le Douarin, and G. Couly The cephalic neural crest provides pericytes and smooth muscle cells to all blood vessels of the face and forebrain Development, January 4, 2001; 128(7): 1059 - 1068. [Abstract] [PDF]
|
|
|
|
|
|
R Kos, M. Reedy, R. Johnson, and C. Erickson The winged-helix transcription factor FoxD3 is important for establishing the neural crest lineage and repressing melanogenesis in avian embryos Development, January 4, 2001; 128(8): 1467 - 1479. [Abstract] [PDF]
|
|
|
|
 |
|
 M. Mina Regulation of Mandibular Growth and Morphogenesis Critical Reviews in Oral Biology & Medicine, January 1, 2001; 12(4): 276 - 300. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Ellies, V Church, P Francis-West, and A Lumsden The WNT antagonist cSFRP2 modulates programmed cell death in the developing hindbrain Development, January 12, 2000; 127(24): 5285 - 5295. [Abstract] [PDF]
|
|
|
|
|
|
P Kulesa, M Bronner-Fraser, and S Fraser In ovo time-lapse analysis after dorsal neural tube ablation shows rerouting of chick hindbrain neural crest Development, January 7, 2000; 127(13): 2843 - 2852. [Abstract] [PDF]
|
|
|
|
|
|
P. Kulesa and S. Fraser In ovo time-lapse analysis of chick hindbrain neural crest cell migration shows cell interactions during migration to the branchial arches Development, January 3, 2000; 127(6): 1161 - 1172. [Abstract] [PDF]
|
|
|
|
|
|
F Mechta-Grigoriou, S Garel, and P Charnay Nab proteins mediate a negative feedback loop controlling Krox-20 activity in the developing hindbrain Development, January 1, 2000; 127(1): 119 - 128. [Abstract] [PDF]
|
|
|
|
|
|
B. Eickholt, S. Mackenzie, A Graham, F. Walsh, and P Doherty Evidence for collapsin-1 functioning in the control of neural crest migration in both trunk and hindbrain regions Development, January 5, 1999; 126(10): 2181 - 2189. [Abstract] [PDF]
|
|
|
|
|
|
F Helmbacher, C Pujades, C Desmarquet, M Frain, F. Rijli, P Chambon, and P Charnay Hoxa1 and Krox-20 synergize to control the development of rhombomere 3 Development, January 12, 1998; 125(23): 4739 - 4748. [Abstract] [PDF]
|
|
|
|
|
|
K Takahashi, G. Nuckolls, O Tanaka, I Semba, I Takahashi, R Dashner, L Shum, and H. Slavkin Adenovirus-mediated ectopic expression of Msx2 in even-numbered rhombomeres induces apoptotic elimination of cranial neural crest cells in ovo Development, January 5, 1998; 125(9): 1627 - 1635. [Abstract] [PDF]
|
|
|
|
|
|
A Grapin-Botton, M. Bonnin, M Sieweke, and N. Le Douarin Defined concentrations of a posteriorizing signal are critical for MafB/Kreisler segmental expression in the hindbrain Development, January 4, 1998; 125(7): 1173 - 1181. [Abstract] [PDF]
|
|
|
|
|
|
T Theil, M Frain, P Gilardi-Hebenstreit, A Flenniken, P Charnay, and D. Wilkinson Segmental expression of the EphA4 (Sek-1) receptor tyrosine kinase in the hindbrain is under direct transcriptional control of Krox-20 Development, January 2, 1998; 125(3): 443 - 452. [Abstract] [PDF]
|
|
|
|
|
|
J. Saldivar, J. Sechrist, C. Krull, S Ruffins, and M Bronner-Fraser Dorsal hindbrain ablation results in rerouting of neural crest migration and changes in gene expression, but normal hyoid development Development, January 7, 1997; 124(14): 2729 - 2739. [Abstract] [PDF]
|
|
|
|
|
|
V Dupe, M Davenne, J Brocard, P Dolle, M Mark, A Dierich, P Chambon, and F. Rijli In vivo functional analysis of the Hoxa-1 3' retinoic acid response element (3'RARE) Development, January 1, 1997; 124(2): 399 - 410. [Abstract] [PDF]
|
|
|
|
|
|
G Kontges and A Lumsden Rhombencephalic neural crest segmentation is preserved throughout craniofacial ontogeny Development, January 10, 1996; 122(10): 3229 - 3242. [Abstract] [PDF]
|
|
|
|
|
|
D Psychoyos and C. Stern Fates and migratory routes of primitive streak cells in the chick embryo Development, January 5, 1996; 122(5): 1523 - 1534. [Abstract] [PDF]
|
|
|
|
|
|
J. Saldivar, C. Krull, R Krumlauf, L Ariza-McNaughton, and M Bronner-Fraser Rhombomere of origin determines autonomous versus environmentally regulated expression of Hoxa-3 in the avian embryo Development, January 3, 1996; 122(3): 895 - 904. [Abstract] [PDF]
|
|
|
|
|
|
S Nonchev, C Vesque, M Maconochie, T Seitanidou, L Ariza-McNaughton, M Frain, H Marshall, M. Sham, R Krumlauf, and P Charnay Segmental expression of Hoxa-2 in the hindbrain is directly regulated by Krox-20 Development, January 2, 1996; 122(2): 543 - 554. [Abstract] [PDF]
|
|
|
|
|
|
J Sechrist, M. Nieto, R. Zamanian, and M Bronner-Fraser Regulative response of the cranial neural tube after neural fold ablation: spatiotemporal nature of neural crest regeneration and up-regulation of Slug Development, January 12, 1995; 121(12): 4103 - 4115. [Abstract] [PDF]
|
|
